Datasheet

生物描述

特异性	Leptin Receptor Rabbit Recombinant mAb detects endogenous levels of total leptin receptor.
背景	Under normal physiological conditions, leptin and the leptin receptor (ObR) regulate the body weight by balancing food intake and energy expenditure. Leptin receptor (ObR) is a single membrane-spanning receptor belonging to the class I cytokine receptor family. Leptin receptor is extensively expressed in many brain regions, including the hypothalamus, the midbrain, and the hindbrain. In addition to the brain, LEPRb is also expressed in peripheral tissues. LEPRb does not contain intrinsic enzymatic activity but bind to a cytoplasmic tyrosine kinase called Janus kinase 2 (JAK2). Leptin stimulates JAK2 activation that subsequently autophosphorylates on multiple tyrosines. JAK2 also phosphorylates LEPRb on three tyrosine residues: Tyr985, Tyr1077, and Tyr1138. Phopho-Tyr985, -Tyr1077, and -Tyr1138 serve as binding sites for additional signaling molecules that contain the Src homology 2 (SH2) domain, and recruit these downstream molecules to the LEPRb-JAK2 complex to allow JAK2 to phosphorylate these effector proteins. Aside from JAK2, the Src tyrosine kinase family members appear also to be involved in mediating leptin signaling independently of JAK2

特异性

Leptin Receptor Rabbit Recombinant mAb detects endogenous levels of total leptin receptor.

背景

Under normal physiological conditions, leptin and the leptin receptor (ObR) regulate the body weight by balancing food intake and energy expenditure. Leptin receptor (ObR) is a single membrane-spanning receptor belonging to the class I cytokine receptor family. Leptin receptor is extensively expressed in many brain regions, including the hypothalamus, the midbrain, and the hindbrain. In addition to the brain, LEPRb is also expressed in peripheral tissues. LEPRb does not contain intrinsic enzymatic activity but bind to a cytoplasmic tyrosine kinase called Janus kinase 2 (JAK2). Leptin stimulates JAK2 activation that subsequently autophosphorylates on multiple tyrosines. JAK2 also phosphorylates LEPRb on three tyrosine residues: Tyr985, Tyr1077, and Tyr1138. Phopho-Tyr985, -Tyr1077, and -Tyr1138 serve as binding sites for additional signaling molecules that contain the Src homology 2 (SH2) domain, and recruit these downstream molecules to the LEPRb-JAK2 complex to allow JAK2 to phosphorylate these effector proteins. Aside from JAK2, the Src tyrosine kinase family members appear also to be involved in mediating leptin signaling independently of JAK2

使用信息

抗体应用

WB,ELISA,ELISA

稀释比例

WB

1:1000

反应性

Human Mouse Rat

MW (kDa)

150kDa

抗体类型

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

Application Data

WB

Validated by Selleck