MacroH2A.1 Rabbit Recombinant mAb Datasheet

生物描述

特异性	MacroH2A.1 Rabbit Recombinant mAb detects endogenous level of total MacroH2A.1.
背景	MacroH2A isoforms are unique H2A histone variants. MacroH2A variants are generally considered transcriptionally repressive in nature due to their association with forms of condensed chromatin such as the inactive X chromosome (Xi), and inactive genes. MacroH2A has been implicated in transcriptional silencing via mechanisms including blocking recruitment of the SWI/SNF nucleosome remodeling complex, repressing p300 and Gal-VP16-driven RNA pol II transcriptional initiation, and modulating Parp-1. MacroH2A1 is alternatively spliced, resulting in two macroH2A1 isoforms, macroH2A1.1 and macroH2A1.2, that differ by only one exon in the macro domain. MacroH2A1 is involved in stem cell reprogramming and cancer progression. It is expressed in nearly all cell types and is implicated in a number of processes including cell cycle, gene regulation, DNA damage repair and senescence.

特异性

MacroH2A.1 Rabbit Recombinant mAb detects endogenous level of total MacroH2A.1.

背景

MacroH2A isoforms are unique H2A histone variants. MacroH2A variants are generally considered transcriptionally repressive in nature due to their association with forms of condensed chromatin such as the inactive X chromosome (Xi), and inactive genes. MacroH2A has been implicated in transcriptional silencing via mechanisms including blocking recruitment of the SWI/SNF nucleosome remodeling complex, repressing p300 and Gal-VP16-driven RNA pol II transcriptional initiation, and modulating Parp-1. MacroH2A1 is alternatively spliced, resulting in two macroH2A1 isoforms, macroH2A1.1 and macroH2A1.2, that differ by only one exon in the macro domain. MacroH2A1 is involved in stem cell reprogramming and cancer progression. It is expressed in nearly all cell types and is implicated in a number of processes including cell cycle, gene regulation, DNA damage repair and senescence.

使用信息

抗体应用

WB, IHC, IF,ELISA,ELISA

稀释比例

WB	IHC	IF
1:1000	1:50	1:50

反应性

Human Mouse Rat

MW (kDa)

40kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

Application Data

WB

Validated by Selleck

IHC

Validated by Selleck

IF

Validated by Selleck