生物描述

特异性

mTOR Rabbit Recombinant mAb detects endogenous levels of total mTOR.

背景

The mammalian target of rapamycin (mTOR) signaling pathway senses and integrates a variety of environmental cues to regulate organismal growth and homeostasis. The pathway regulates many major cellular processes and is implicated in an increasing number of pathological conditions, including cancer, obesity, type 2 diabetes, and neurodegeneration. mTOR is an atypical serine/threonine protein kinase that belongs to the phosphoinositide 3-kinase (PI3K)-related kinase family and interacts with several proteins to form two distinct complexes named mTOR complex 1 (mTORC1) and 2 (mTORC2). The mTOR-containing complexes have different sensitivities to rapamycin as well as upstream inputs and downstream outputs. Both mTOR complexes are large, with mTORC1 having six and mTORC2 seven known protein components. They share the catalytic mTOR subunit, and also mammalian lethal with sec-13 (mLST8, also known as GβL), DEP domain containing mTOR-interacting protein (DEPTOR), and he Tti1/Tel2 complex. Raptor and PRAS40 are specific to mTORC1, while rictor, mSin1 and protor1/2 are only part of mTORC2.

使用信息

抗体应用	WB, IHC,ELISA,ELISA
稀释比例	WB IHC 1:1000 1:50
反应性	Human Mouse Rat
MW (kDa)	289kDa
抗体类型
储存液配方	10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.
储存条件 (自收到货起)	Store at –20°C.

文献参考

[1] Mathieu Laplante, et al. Cell. 2012, 149(2): 274–293.

Application Data

WB

IHC