Datasheet

生物描述

特异性	Mutant p53 Rabbit Recombinant mAb detects endogenous levels of total Mutant p53.
背景	Mutant p53 protein often accumulates to very high levels in tumors, contributing to malignant progression. Mutant p53 in general can no longer recognize and bind to p53 DNA binding elements and lose the transcriptional activity towards wild type p53-regulated target genes. However, many tumor-associated mutant p53 proteins are mainly localized in the nucleus and can regulate the transcription of some genes through mechanisms different from wild type p53. Mutant p53 can bind to TAp63 and TAp73 and inhibit their transcriptional activities. TAp63 and TAp73 are two important p53 family members which can regulate some of wild type p53 target genes and compensate for some of p53 tumor suppressive functions. Mutant p53 can also interact with other transcriptional factors and cofactors, including NF-Y, SREBP, VDR, Sp1, ETS2 and NRF2, and enhance or decrease their transcription activities to promote tumor progression. Mutant p53 can also participate in the regulation of chromatin structure to regulate the expression of some genes. It can recognize and bind with high affinity to DNA regions of matrix attachment region DNA elements (MARs), which have high potential for DNA base-unpairing. In addition to its nuclear function, mutant p53 has cytoplasmic function: it promotes translocation of glucose transporter 1 (GLUT1) to plasma membrane to stimulate the Warburg effect, a characterized metabolic change in tumor cells and a key contributor to tumor development.

特异性

Mutant p53 Rabbit Recombinant mAb detects endogenous levels of total Mutant p53.

背景

Mutant p53 protein often accumulates to very high levels in tumors, contributing to malignant progression. Mutant p53 in general can no longer recognize and bind to p53 DNA binding elements and lose the transcriptional activity towards wild type p53-regulated target genes. However, many tumor-associated mutant p53 proteins are mainly localized in the nucleus and can regulate the transcription of some genes through mechanisms different from wild type p53. Mutant p53 can bind to TAp63 and TAp73 and inhibit their transcriptional activities. TAp63 and TAp73 are two important p53 family members which can regulate some of wild type p53 target genes and compensate for some of p53 tumor suppressive functions. Mutant p53 can also interact with other transcriptional factors and cofactors, including NF-Y, SREBP, VDR, Sp1, ETS2 and NRF2, and enhance or decrease their transcription activities to promote tumor progression. Mutant p53 can also participate in the regulation of chromatin structure to regulate the expression of some genes. It can recognize and bind with high affinity to DNA regions of matrix attachment region DNA elements (MARs), which have high potential for DNA base-unpairing. In addition to its nuclear function, mutant p53 has cytoplasmic function: it promotes translocation of glucose transporter 1 (GLUT1) to plasma membrane to stimulate the Warburg effect, a characterized metabolic change in tumor cells and a key contributor to tumor development.

使用信息

抗体应用

WB, IF,ELISA,ELISA

稀释比例

WB	IF
1:1000	1:50

反应性

Human

MW (kDa)

53kDa

抗体类型

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

[1] Xuetian Yue, et al. J Mol Biol. 2017, 429(11): 1595–1606.

Application Data

WB

Validated by Selleck

IF

Validated by Selleck