NF-κB (p105/p50) Rabbit Recombinant mAb Datasheet

生物描述

特异性	NF-κB (p105/p50) Rabbit Recombinant mAb detects endogenous level of total NF-κB (p105/p50).
背景	The NF-κB family is composed of five mammalian members: RelA/p65, RelB, c-Rel, NF-κB1 p105/p50, and NF-κB2 p100/p52. Active forms of NF-κB subunit p50 and p52 are produced by ubiquitin-dependent proteolytic process of the COOH-terminal domains of NF-κB1 p105 and NF-κB2 p100, respectively. In most cells, NF-κB subunit forms homodimers or heterodimers and sequestered by IκB in cytoplasm. The activation of NF-κB dimers in response to stimuli results in nuclear translocation of the dimers, which then regulate a wide variety of gene expression.

特异性

NF-κB (p105/p50) Rabbit Recombinant mAb detects endogenous level of total NF-κB (p105/p50).

背景

The NF-κB family is composed of five mammalian members: RelA/p65, RelB, c-Rel, NF-κB1 p105/p50, and NF-κB2 p100/p52. Active forms of NF-κB subunit p50 and p52 are produced by ubiquitin-dependent proteolytic process of the COOH-terminal domains of NF-κB1 p105 and NF-κB2 p100, respectively. In most cells, NF-κB subunit forms homodimers or heterodimers and sequestered by IκB in cytoplasm. The activation of NF-κB dimers in response to stimuli results in nuclear translocation of the dimers, which then regulate a wide variety of gene expression.

使用信息

抗体应用

WB, IHC,ELISA,ELISA

稀释比例

WB	IHC
1:1000	1:50

反应性

Human Mouse Rat

MW (kDa)

50kDa/105kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

[1] Kai Gao, et al. Cancer Research. 2006, 66(17):8382-8388.

Application Data

WB

Validated by Selleck

IHC

Validated by Selleck