PDGF Receptor beta Rabbit Recombinant mAb Datasheet

生物描述

特异性	PDGF Receptor beta Rabbit Recombinant mAb detects endogenous level of total PDGF Receptor beta.
背景	The PDGF isoforms bind two distinct class III receptor tyrosine kinases, PDGFRα and PDGFRβ, which are primarily located in vascular endothelial cells, fibroblasts and Kupffer cells. Binding of the ligand leads to autophosphorylation of the receptors on tyrosine residues and this event induces activation of several signaling molecules. PDGFRα has high affinity for PDGF-A, -B, and -C associated with the early hematopoietic system and vascularization, whereas PDGFRβ has high affinity for PDGF-B and –D. Ligand binding to receptors induces receptor dimerization, which leads to activation of the intrinsic tyrosine kinase domain and subsequent recruitment of SH-2-domain-containing signaling proteins. Finally, activation of these pathways leads to cellular responses, like proliferation and migration.

特异性

PDGF Receptor beta Rabbit Recombinant mAb detects endogenous level of total PDGF Receptor beta.

背景

The PDGF isoforms bind two distinct class III receptor tyrosine kinases, PDGFRα and PDGFRβ, which are primarily located in vascular endothelial cells, fibroblasts and Kupffer cells. Binding of the ligand leads to autophosphorylation of the receptors on tyrosine residues and this event induces activation of several signaling molecules. PDGFRα has high affinity for PDGF-A, -B, and -C associated with the early hematopoietic system and vascularization, whereas PDGFRβ has high affinity for PDGF-B and –D. Ligand binding to receptors induces receptor dimerization, which leads to activation of the intrinsic tyrosine kinase domain and subsequent recruitment of SH-2-domain-containing signaling proteins. Finally, activation of these pathways leads to cellular responses, like proliferation and migration.

使用信息

抗体应用

WB, IHC,ELISA,ELISA

稀释比例

WB	IHC
1:1000	1:50

反应性

Human Mouse Rat

MW (kDa)

170kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

Application Data

WB

Validated by Selleck

IHC

Validated by Selleck