PE Mouse CD39 Antibody [24DMS1]

目录号:G2882

打印

生物描述

特异性
背景 CD39是一种具有两个跨膜结构域的整合膜蛋白,形成同源四聚体结构。它被认为是免疫系统中最突出的外切酶,主要通过将有毒的胞外ATP转化为ADP或AMP来发挥作用。CD39与CD73协同水解ATP,在朗格汉斯细胞上已被充分鉴定。最初在活化的淋巴细胞上发现,CD39表达于多种免疫细胞,包括外周血B细胞、单核细胞和T细胞克隆。它也存在于部分T细胞、B细胞和树突状细胞亚群中,在单核细胞和粒细胞上表达较弱。CD39细胞表面抗原是一种70-100 kDa的分子,其表达在T细胞上被诱导,在B细胞上作为晚期活化抗原而增加。

使用信息

抗体应用 FCM 推荐用量 0.2 μg (1 μL) / million cells in 100 µL volume
反应性 Mouse
抗体类型 Rat MW 70-100 kDa
储存液配方 Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
储存条件
(自收到货起)
Store at 4°C in the dark to avoid freeze-thaw cycles

Application Data

FCM

Validated by Selleck

  • Surface staining of splenic lymphocytes from C57BL/6 mice using PE Mouse CD39 Antibody [24DMS1] (Selleck Cat.: G2882) and CD4 Antibody, after washing, permeabilized using Foxp3/Transcription Factor Staining Buffer Set (Permeabilization Reagent), and after permeabilization stained for intranuclear staining using anti-mouse Foxp3 (green, with red blank control). Analyzed data are derived from CD4+ Foxp3+ cells or CD4+ Foxp3- cells.