PHD1/prolyl hydroxylase Rabbit Recombinant mAb Datasheet

生物描述

特异性	PHD1/prolyl hydroxylase Rabbit Recombinant mAb detects endogenous level of total PHD1/prolyl hydroxylase.
背景	Prolyl hydroxylases (PHDs) play an important role in regulating HIF-α isoform stability. Three PHD isoforms have been identified in mammals (PHD1, PHD2, and PHD3) and are ubiquitously expressed in most tissues. In normoxic conditions, PHDs act as oxygen-sensing enzymes and promote hydroxylation of HIF-α subunits, allowing recognition by the protein von Hippel-Lindau (pVhl) ubiquitin ligase complex and subsequent proteasomal degradation. PHD activity is reduced during hypoxia, leading to stabilization of HIF-α subunits and their subsequent translocation to the nucleus where they can dimerize with HIF-β, subsequently triggering the transcription of specific target genes.

特异性

PHD1/prolyl hydroxylase Rabbit Recombinant mAb detects endogenous level of total PHD1/prolyl hydroxylase.

背景

Prolyl hydroxylases (PHDs) play an important role in regulating HIF-α isoform stability. Three PHD isoforms have been identified in mammals (PHD1, PHD2, and PHD3) and are ubiquitously expressed in most tissues. In normoxic conditions, PHDs act as oxygen-sensing enzymes and promote hydroxylation of HIF-α subunits, allowing recognition by the protein von Hippel-Lindau (pVhl) ubiquitin ligase complex and subsequent proteasomal degradation. PHD activity is reduced during hypoxia, leading to stabilization of HIF-α subunits and their subsequent translocation to the nucleus where they can dimerize with HIF-β, subsequently triggering the transcription of specific target genes.

使用信息

抗体应用

WB,ELISA,ELISA

稀释比例

WB

1:1000

反应性

Human Mouse Rat

MW (kDa)

44kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

[1] Thomas A, et al. Sci Rep. 2016, 6:24618.

Application Data

WB

Validated by Selleck