Datasheet

生物描述

特异性	Phospho-AMPK alpha 2 (S491) Rabbit Recombinant mAb detects endogenous levels of phosphorylated AMPK alpha 2 (S491).
背景	AMPK is an evolutionarily conserved serine/threonine kinase that was originally identified as the key player in maintaining cellular energy homeostasis. AMPK regulates diverse metabolic and physiological processes and is dysregulated in major chronic diseases, such as obesity, inflammation, diabetes and cancer. AMPK is a heterotrimeric complex containing one catalytic α-subunit and two regulatory β- and γ-subunits. In mammals, AMPK α- and β-subunits have two isoforms each, and AMPK γ-subunit has three isoforms. In addition to phosphorylation on the critical T172 residue, a number of secondary phosphorylation sites on the α and β subunits have been identified. Phosphorylation at these sites may regulate AMPK activation. For example, α1 S485 and α2 S491 phosphorylation is correlated with decreased AMPK activity in cardiac tissue in response to insulin treatment. α2 S491 phosphorylation by p70S6K kinase, acting downstream of PI3K-AKT, is found to underlie AMPK inhibition in the hypothalamus.

特异性

Phospho-AMPK alpha 2 (S491) Rabbit Recombinant mAb detects endogenous levels of phosphorylated AMPK alpha 2 (S491).

背景

AMPK is an evolutionarily conserved serine/threonine kinase that was originally identified as the key player in maintaining cellular energy homeostasis. AMPK regulates diverse metabolic and physiological processes and is dysregulated in major chronic diseases, such as obesity, inflammation, diabetes and cancer. AMPK is a heterotrimeric complex containing one catalytic α-subunit and two regulatory β- and γ-subunits. In mammals, AMPK α- and β-subunits have two isoforms each, and AMPK γ-subunit has three isoforms. In addition to phosphorylation on the critical T172 residue, a number of secondary phosphorylation sites on the α and β subunits have been identified. Phosphorylation at these sites may regulate AMPK activation. For example, α1 S485 and α2 S491 phosphorylation is correlated with decreased AMPK activity in cardiac tissue in response to insulin treatment. α2 S491 phosphorylation by p70S6K kinase, acting downstream of PI3K-AKT, is found to underlie AMPK inhibition in the hypothalamus.

使用信息

抗体应用

WB,ELISA,ELISA

稀释比例

WB

1:1000

反应性

Human Mouse Rat

MW (kDa)

62kDa

抗体类型

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

Application Data

WB

Validated by Selleck