Datasheet

生物描述

特异性	Phospho-Cdk2 (Y15) Rabbit Recombinant mAb detects endogenous levels of phosphorylated Cdk2 (Y15).
背景	Cyclin-dependent kinases (CDK) play a central role in the control of the eukaryotic cell division cycle. The critical CDK/cyclin complexes for the cell division are CDK2/cyclin E, driving a cell across the G1/S-phase border; CDK2/cyclin A, mediating DNA replication; and CDK1/cyclin B, controlling the entry into mitosis. Monomeric CDK2 is inactive and its activation requires binding to a cyclin (cyclin E at the G1/S transition, cyclin A during the S phase). The CDK2/cyclin complex is recognized by multiple protein kinases, and it results in phosphorylations on T14, Y15, and T160 in CDK2. Phosphorylation at Y15 is an inhibitory phosphorylation, which leads to inactivation of CDK2. The amino acid residue Y15 and to a lesser extent T14 are phosphorylated by human Wee1Hu. This inhibitory phosphorylation is independent of previous cyclin binding. Inhibitory phosphorylation likely precedes the activating T160 phosphorylation by CAK (CDK7/Cyclin H) because activatory phosphorylation requires cyclin binding. The overphosphorylated complex is inactive and subsequent dephosphorylation of T14 and Y15 by CDC25 results in activation.

特异性

Phospho-Cdk2 (Y15) Rabbit Recombinant mAb detects endogenous levels of phosphorylated Cdk2 (Y15).

背景

Cyclin-dependent kinases (CDK) play a central role in the control of the eukaryotic cell division cycle. The critical CDK/cyclin complexes for the cell division are CDK2/cyclin E, driving a cell across the G1/S-phase border; CDK2/cyclin A, mediating DNA replication; and CDK1/cyclin B, controlling the entry into mitosis. Monomeric CDK2 is inactive and its activation requires binding to a cyclin (cyclin E at the G1/S transition, cyclin A during the S phase). The CDK2/cyclin complex is recognized by multiple protein kinases, and it results in phosphorylations on T14, Y15, and T160 in CDK2. Phosphorylation at Y15 is an inhibitory phosphorylation, which leads to inactivation of CDK2. The amino acid residue Y15 and to a lesser extent T14 are phosphorylated by human Wee1Hu. This inhibitory phosphorylation is independent of previous cyclin binding. Inhibitory phosphorylation likely precedes the activating T160 phosphorylation by CAK (CDK7/Cyclin H) because activatory phosphorylation requires cyclin binding. The overphosphorylated complex is inactive and subsequent dephosphorylation of T14 and Y15 by CDC25 results in activation.

使用信息

抗体应用

WB,ELISA,ELISA

稀释比例

WB

1:1000

反应性

Human Mouse Rat

MW (kDa)

34kDa

抗体类型

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

[1] Iveta Bártová, et al. Protein Sci. 2004, 13(6): 1449-1457.

Application Data

WB

Validated by Selleck