Datasheet

生物描述

特异性	Phospho-GSK3 (alpha+beta)(Y216+Y279) Rabbit Recombinant mAb detects endogenous levels of phosphorylated GSK3 (alpha+beta)(Y216+Y279).
背景	Glycogen synthase kinase-3 (GSK-3) is a multifunctional serine/threonine kinase found in all eukaryotes. The enzyme is a key regulator of numerous signalling pathways, including cellular responses to Wnt, receptor tyrosine kinases and G protein-coupled receptors and is involved in a wide swathe of cellular processes ranging from glycogen metabolism to cell cycle regulation and proliferation. GSK-3 is unusual in that it is usually constitutively active in cells and is regulated through inhibition of its activity. The activities of GSK-3 are positively regulated by phosphorylation of Tyr279 and Tyr216 for the α- and β-isoforms, respectively. Phosphorylation of Tyr279 in GSK-3α and Tyr216 in GSK-3β has been reported to facilitate GSK-3 activity by inducing rotation of the tyrosine side chain, which moves out of the substrate-binding groove, promoting substrate accessibility.

特异性

Phospho-GSK3 (alpha+beta)(Y216+Y279) Rabbit Recombinant mAb detects endogenous levels of phosphorylated GSK3 (alpha+beta)(Y216+Y279).

背景

Glycogen synthase kinase-3 (GSK-3) is a multifunctional serine/threonine kinase found in all eukaryotes. The enzyme is a key regulator of numerous signalling pathways, including cellular responses to Wnt, receptor tyrosine kinases and G protein-coupled receptors and is involved in a wide swathe of cellular processes ranging from glycogen metabolism to cell cycle regulation and proliferation. GSK-3 is unusual in that it is usually constitutively active in cells and is regulated through inhibition of its activity. The activities of GSK-3 are positively regulated by phosphorylation of Tyr279 and Tyr216 for the α- and β-isoforms, respectively. Phosphorylation of Tyr279 in GSK-3α and Tyr216 in GSK-3β has been reported to facilitate GSK-3 activity by inducing rotation of the tyrosine side chain, which moves out of the substrate-binding groove, promoting substrate accessibility.

使用信息

抗体应用

WB,ELISA,ELISA

稀释比例

WB

1:1000

反应性

Human Mouse Rat

MW (kDa)

51kDa/46kDa

抗体类型

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

Application Data

WB

Validated by Selleck