Phospho-LAT (Y191) Rabbit Recombinant mAb Datasheet

生物描述

特异性	Phospho-LAT (Y191) Rabbit Recombinant mAb detects endogenous level of total Phospho-LAT (Y191).
背景	The adapter molecule LAT (Linker for activation of T cells) is a nucleating site for multiprotein signaling complexes that are vital for the function and differentiation of T cells. LAT is expressed in T cells and in a limited number of other immune cell types (mast cells, natural killer cells, megakaryocytes, platelets, and immature B cells). LAT facilitates the recruitment of many signaling proteins to the plasma membrane where it links receptors, tyrosine kinases and their substrates and other effector molecules together, functioning as a critical activator of T cells. Structurally, LAT is a type III transmembrane protein. LAT is rapidly phosphorylated on tyrosine residues upon TCR engagement. LAT Y171 and Y191 contribute to PLC-γ1 binding and that all four distal LAT tyrosines (132, 171, 191, and 226) were required for optimal PLC-γ1 phosphorylation and downstream calcium influx. Grb2 family members bind Y171, Y191, and Y226 of phosphorylated LAT and recruit a number of signaling proteins to the LAT complex.

特异性

Phospho-LAT (Y191) Rabbit Recombinant mAb detects endogenous level of total Phospho-LAT (Y191).

背景

The adapter molecule LAT (Linker for activation of T cells) is a nucleating site for multiprotein signaling complexes that are vital for the function and differentiation of T cells. LAT is expressed in T cells and in a limited number of other immune cell types (mast cells, natural killer cells, megakaryocytes, platelets, and immature B cells). LAT facilitates the recruitment of many signaling proteins to the plasma membrane where it links receptors, tyrosine kinases and their substrates and other effector molecules together, functioning as a critical activator of T cells. Structurally, LAT is a type III transmembrane protein. LAT is rapidly phosphorylated on tyrosine residues upon TCR engagement. LAT Y171 and Y191 contribute to PLC-γ1 binding and that all four distal LAT tyrosines (132, 171, 191, and 226) were required for optimal PLC-γ1 phosphorylation and downstream calcium influx. Grb2 family members bind Y171, Y191, and Y226 of phosphorylated LAT and recruit a number of signaling proteins to the LAT complex.

使用信息

抗体应用

WB, IHC,ELISA,ELISA

稀释比例

WB	IHC
1:1000	1:50

反应性

Human Mouse Rat

MW (kDa)

38kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

[1] Balagopalan L, et al. Cold Spring Harb Perspect Biol. 2010, 2(8):a005512.

Application Data

WB

Validated by Selleck

IHC

Validated by Selleck