Phospho-MSK1 (S376) Rabbit Recombinant mAb Datasheet

生物描述

特异性	Phospho-MSK1 (S376) Rabbit Recombinant mAb detects endogenous level of total phospho-MSK1 (S376).
背景	MSK1 is a dual kinase domain protein that acts downstream of the ERK1/2 and p38 MAPK signalling pathways in cells. MSK1, and its related isoform MSK2, phosphorylate the transcription factors CREB and ATF1, and the chromatin proteins histone H3 and HMGN1 in response to either mitogenic stimulation or cellular stress. MSK1 activity is tightly regulated in cells and activation requires the phosphorylation of MSK1 by either ERK1/2 or p38α. This results in activation of the C-terminal kinase domain, which then phosphorylates further sites in MSK1 leading to the activation of the N-terminal kinase domain and phosphorylation of substrates. Ser376 is an autophosphorylation site in the linker region. Ser376 appears to be essential for MSK activation as its mutation to alanine prevents MSK1 activation.

特异性

Phospho-MSK1 (S376) Rabbit Recombinant mAb detects endogenous level of total phospho-MSK1 (S376).

背景

MSK1 is a dual kinase domain protein that acts downstream of the ERK1/2 and p38 MAPK signalling pathways in cells. MSK1, and its related isoform MSK2, phosphorylate the transcription factors CREB and ATF1, and the chromatin proteins histone H3 and HMGN1 in response to either mitogenic stimulation or cellular stress. MSK1 activity is tightly regulated in cells and activation requires the phosphorylation of MSK1 by either ERK1/2 or p38α. This results in activation of the C-terminal kinase domain, which then phosphorylates further sites in MSK1 leading to the activation of the N-terminal kinase domain and phosphorylation of substrates. Ser376 is an autophosphorylation site in the linker region. Ser376 appears to be essential for MSK activation as its mutation to alanine prevents MSK1 activation.

使用信息

抗体应用

WB,ELISA,ELISA

稀释比例

WB

1:1000

反应性

Human Rat

MW (kDa)

90kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

[1] McCoy CE, et al. Biochem J. 2007, 402(3):491-501.

Application Data

WB

Validated by Selleck