Phospho-Nrf2 (S40) Rabbit Recombinant mAb

生物描述

特异性

Phospho-Nrf2 (S40) Rabbit Recombinant mAb detects endogenous level of total Phospho-Nrf2 (S40).

背景

Nrf2 is the key transcription factor regulating the antioxidant response. Nrf2 signaling is regulated by compartmental segregation. Under basal conditions, Nrf2 is found mainly sequestered in the cytoplasm. When challenged by oxidative stress derived from accumulation of ROS or reactive nitrogen species (RNS), Nrf2 can quickly translocate into the nucleus and elicit the antioxidant response. At least four components in combine, namely Nrf2, Keap1 (Kelch-like ECH-associated protein 1), a group of small musculoaponeurotic fibrosarcoma (Maf) proteins and a cis-acting enhancer called antioxidant response element (ARE) or electrophile responsive element (EpRE), are essential for the antioxidant response. Nrf2 signaling is repressed by Keap1 at basal condition and induced by oxidative stress. PKC phosphorylation of the Serine-40 (S40) residue of Nrf2 was reported to facilitate Keap1/Nrf2 dissociation and Nrf2 nuclear translocation.

使用信息

抗体应用	WB, IF,ELISA,ELISA
稀释比例	WB IF 1:1000 1:50
反应性	Human
MW (kDa)	90kDa
抗体类型	Rabbit
储存液配方	10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.
储存条件 (自收到货起)	Store at –20°C.

文献参考

[1] Li W, et al. Mol Carcinog. 2009, 48(2):91-104.

Application Data

WB

IF