生物描述

特异性

Phospho-PKC zeta (T560) Rabbit Recombinant mAb detects endogenous levels of phosphorylated PKC zeta (T560).

背景

Protein kinase C-zeta (PKCζ) is an atypical isoform of the PKC family of protein serine/threonine kinases. Unlike the conventional and the novel isoforms, the atypical PKCs neither respond to second messenger diacylglycerol (DAG) nor bind calcium. They are, however, sensitive to phosphoinositide 3-kinase (PI3-K) lipid product phosphatidylinositol (3,4,5)-trisphosphate (PIP3) and are activated by 3-phosphoinositide dependent protein kinase-1 (PDK1) in a PIP3-enhanced manner. PKCζ, in addition, can be activated by phospholipase D2 (PLD2) via protein-protein interaction. Phosphorylation of the threonine 410 residue present in the activation loop and autophosphorylation of threonine 560 residue present in the turn motif, of the kinase domain of the enzyme, have been shown to be important for its kinase activity. However, The T560 autophosphorylation signal alone might not be sufficient to maintain its full kinase activity.

使用信息

抗体应用	IHC, WB,ELISA,ELISA
稀释比例	WB IHC 1:1000 1:50
反应性	Human Mouse Rat
MW (kDa)	68kDa
抗体类型
储存液配方	10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.
储存条件 (自收到货起)	Store at –20°C.

文献参考

[1] Mayanglambam A, et al. Biochem Pharmacol. 2011, 82(5):505-13.

Application Data

WB

IHC