Phospho-RPA2 (T21) Rabbit Recombinant mAb Datasheet

生物描述

特异性	Phospho-RPA2 (T21) Rabbit Recombinant mAb detects endogenous level of total phospho-RPA2 (T21).
背景	One factor that plays essential roles both during DNA replication and in the repair- and recombination-mediated recovery from damage is replication protein A (RPA), the eukaryotic single-stranded (ss) DNA-binding protein. RPA is a heterotrimeric protein consisting, in mammalian cells, of ∼70- (RPA1), 30- (RPA2), and 14 (RPA3)-kDa subunits. During DNA replication, RPA acts at the fork, stabilizing ssDNA and facilitating nascent strand synthesis by the replicative DNA polymerases. Under DNA-damaging conditions, RPA-ssDNA complexes act to recruit and activate a key checkpoint mediator consisting of the ATR and ATRIP (ATR-interacting protein) protein-kinase complex. At DNA damage-dependent nuclear foci, RPA interacts with repair and recombination components to process double-strand DNA breaks and other lesions. RPA activity is regulated by various stress conditions. In particular, heat shock, exposure to UV radiation, and treatment with DNA-alkylating agents each cause the generation of an RPA species that is unable to support DNA replication in vitro. Two of the RPA2 sites (S23 and S29) are phosphorylated in a cell cycle-dependent manner by cyclin-cdk2 complexes. At least five of the other seven (S4, S8, S11, S12, S13, T21, and S33) can be phosphorylated in response to UV irradiation.

特异性

Phospho-RPA2 (T21) Rabbit Recombinant mAb detects endogenous level of total phospho-RPA2 (T21).

背景

One factor that plays essential roles both during DNA replication and in the repair- and recombination-mediated recovery from damage is replication protein A (RPA), the eukaryotic single-stranded (ss) DNA-binding protein. RPA is a heterotrimeric protein consisting, in mammalian cells, of ∼70- (RPA1), 30- (RPA2), and 14 (RPA3)-kDa subunits. During DNA replication, RPA acts at the fork, stabilizing ssDNA and facilitating nascent strand synthesis by the replicative DNA polymerases. Under DNA-damaging conditions, RPA-ssDNA complexes act to recruit and activate a key checkpoint mediator consisting of the ATR and ATRIP (ATR-interacting protein) protein-kinase complex. At DNA damage-dependent nuclear foci, RPA interacts with repair and recombination components to process double-strand DNA breaks and other lesions. RPA activity is regulated by various stress conditions. In particular, heat shock, exposure to UV radiation, and treatment with DNA-alkylating agents each cause the generation of an RPA species that is unable to support DNA replication in vitro. Two of the RPA2 sites (S23 and S29) are phosphorylated in a cell cycle-dependent manner by cyclin-cdk2 complexes. At least five of the other seven (S4, S8, S11, S12, S13, T21, and S33) can be phosphorylated in response to UV irradiation.

使用信息

抗体应用

WB,ELISA,ELISA

稀释比例

WB

1:1000

反应性

Human Mouse Rat

MW (kDa)

32kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

[1] Vitaly M Vassin, et al. Mol Cell Biol. 2004, 24(5): 1930-1943.

Application Data

WB

Validated by Selleck