Phospho-RSK1 (S380) Rabbit Recombinant mAb Datasheet

生物描述

特异性	Phospho-RSK1 (S380) Rabbit Recombinant mAb detects endogenous level of total phospho-RSK1 (S380).
背景	Ribosomal S6 kinase 1 (RSK1) belongs to a family of proteins with two kinase domains. Following activation in the cytoplasm by extracellular signal-regulated kinases (ERK1/2), it mediates the cell-proliferative, cell-growth, and survival-promoting actions of a number of growth factors and other agonists. Among the four isoforms of RSKs that are products of different genes, RSK1, RSK2, and RSK3 share significant sequence similarity; RSK4 is longer than the other isoforms and may also be functionally different. Despite their similarity, RSK1, RSK2, and RSK3 are not functionally redundant. The activation of RSK1 depends on the docking of ERK1/2 to the C-terminus. Following docking onto RSK1, ERK1/2 phosphorylates T573 in the activation loop of the C-terminal kinase (CTK), as well as T359 and S363 in the linker region that joins the N-terminal kinase (NTK) and CTK. Phosphorylation of T573 activates the CTK, and this domain can then phosphorylate S380 in the linker region. Phosphorylation of S380 provides a docking site for phosphoinositide-dependent kinase 1 (PDK1), which can then phosphorylate S221 in the activation loop of the NTK and permits RSK1 to phosphorylate its downstream substrates. On activation of RSK1 in the cytoplasm and at the plasma membrane, the fully active RSK1 localizes to the nucleus.

特异性

Phospho-RSK1 (S380) Rabbit Recombinant mAb detects endogenous level of total phospho-RSK1 (S380).

背景

Ribosomal S6 kinase 1 (RSK1) belongs to a family of proteins with two kinase domains. Following activation in the cytoplasm by extracellular signal-regulated kinases (ERK1/2), it mediates the cell-proliferative, cell-growth, and survival-promoting actions of a number of growth factors and other agonists. Among the four isoforms of RSKs that are products of different genes, RSK1, RSK2, and RSK3 share significant sequence similarity; RSK4 is longer than the other isoforms and may also be functionally different. Despite their similarity, RSK1, RSK2, and RSK3 are not functionally redundant. The activation of RSK1 depends on the docking of ERK1/2 to the C-terminus. Following docking onto RSK1, ERK1/2 phosphorylates T573 in the activation loop of the C-terminal kinase (CTK), as well as T359 and S363 in the linker region that joins the N-terminal kinase (NTK) and CTK. Phosphorylation of T573 activates the CTK, and this domain can then phosphorylate S380 in the linker region. Phosphorylation of S380 provides a docking site for phosphoinositide-dependent kinase 1 (PDK1), which can then phosphorylate S221 in the activation loop of the NTK and permits RSK1 to phosphorylate its downstream substrates. On activation of RSK1 in the cytoplasm and at the plasma membrane, the fully active RSK1 localizes to the nucleus.

使用信息

抗体应用

WB,ELISA,ELISA

稀释比例

WB

1:1000

反应性

Human Mouse Rat

MW (kDa)

90kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

[1] Xianlong Gao, et al. Mol Biol Cell. 2012, 23(3): 503–515.

Application Data

WB

Validated by Selleck