Datasheet

生物描述

特异性	Phospho-Smad2 (S255) Rabbit Recombinant mAb detects endogenous levels of phosphorylated Smad2 (S255).
背景	In the TGF-β pathway, Smad2 and Smad3 are receptor-regulated effector proteins (R-Smads), which are phosphorylated by the activated TβRI, resulting in R-Smad nuclear accumulation. Mitogens and hyperactive Ras induce ERK-mediated linker phosphorylation of Smad2 at Ser245/250/255/Thr220 and Smad3 at Ser204/208/Thr179. pSmad2L (Ser255) serves as STAT3 co-activator in combination with p300, a co-activator of various transcription factors including both Smads and STAT3, whereas unphosphorylated Smad3C serves as the STAT3 co-repressor in combination with PIAS3, a negative regulator of STAT3-induced transcription.

特异性

Phospho-Smad2 (S255) Rabbit Recombinant mAb detects endogenous levels of phosphorylated Smad2 (S255).

背景

In the TGF-β pathway, Smad2 and Smad3 are receptor-regulated effector proteins (R-Smads), which are phosphorylated by the activated TβRI, resulting in R-Smad nuclear accumulation. Mitogens and hyperactive Ras induce ERK-mediated linker phosphorylation of Smad2 at Ser245/250/255/Thr220 and Smad3 at Ser204/208/Thr179. pSmad2L (Ser255) serves as STAT3 co-activator in combination with p300, a co-activator of various transcription factors including both Smads and STAT3, whereas unphosphorylated Smad3C serves as the STAT3 co-repressor in combination with PIAS3, a negative regulator of STAT3-induced transcription.

使用信息

抗体应用

WB,ELISA,ELISA

稀释比例

WB

1:1000

反应性

Human Mouse Rat

MW (kDa)

52kDa

抗体类型

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

Application Data

WB

Validated by Selleck