Datasheet

生物描述

特异性	Phospho-STAT1 (S727) Rabbit Recombinant mAb detects endogenous levels of phosphorylated STAT1 (S727).
背景	Serine phosphorylation has generally been considered indispensable for full transcriptional activity of STAT proteins. STAT1 is a cytoplasmic protein that, upon awakening from dormancy by signaling from IFNs and other factors, activates numerous genes designed, in principle, to defend the host. STAT1 acts as a tumor promoter in leukemia. The activity of Stat1 is regulated by phosphorylation on Tyr701 by Janus kinases (JAKs), important for its dimerization, translocation to the nucleus, and binding to DNA. A second phosphorylation, of Ser727, is required for transcriptional responses to certain inducers, eg, IFN-γ, but not to others, eg, IFN-α. In accordance with this requirement, both Stat1 Tyr701 and Ser727 phosphorylation are necessary for growth arrest in response to IFN-γ, whereas IFN-α—induced growth arrest requires only Tyr701 phosphorylation.

特异性

Phospho-STAT1 (S727) Rabbit Recombinant mAb detects endogenous levels of phosphorylated STAT1 (S727).

背景

Serine phosphorylation has generally been considered indispensable for full transcriptional activity of STAT proteins. STAT1 is a cytoplasmic protein that, upon awakening from dormancy by signaling from IFNs and other factors, activates numerous genes designed, in principle, to defend the host. STAT1 acts as a tumor promoter in leukemia. The activity of Stat1 is regulated by phosphorylation on Tyr701 by Janus kinases (JAKs), important for its dimerization, translocation to the nucleus, and binding to DNA. A second phosphorylation, of Ser727, is required for transcriptional responses to certain inducers, eg, IFN-γ, but not to others, eg, IFN-α. In accordance with this requirement, both Stat1 Tyr701 and Ser727 phosphorylation are necessary for growth arrest in response to IFN-γ, whereas IFN-α—induced growth arrest requires only Tyr701 phosphorylation.

使用信息

抗体应用

WB,ELISA,ELISA

稀释比例

WB

1:1000

反应性

Human Mouse Rat

MW (kDa)

87kDa

抗体类型

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

Application Data

WB

Validated by Selleck