PI3 Kinase Class 3 Rabbit Recombinant mAb Datasheet

生物描述

特异性	PI3 Kinase Class 3 Rabbit Recombinant mAb detects endogenous level of total PI3 Kinase Class 3.
背景	PI3Ks are spatially regulated and multifunctional, and together, act at nearly all membranes in the cell to regulate a wide range of signaling, membrane trafficking and metabolic processes. There are three different PI3K classes (I, II and III) and different isoforms within each class. All three PI3K classes (I, II and III) phosphorylate the 3′-position hydroxyl of the D-myo-inositol head group to generate specific phosphoinositide forms. The PI3K family appears restricted to eukaryotes, and only the class III PI3K is conserved from yeast to human. Unlike class I and II PI 3-kinases, class III PI3K can phosphorylate only PtdIns and is thus a PtdIns 3-kinase. The single class 3 PI3K, that is Vps34, binds to the adaptor protein Vps15 which is N-terminally myristoylated and regulates the intracellular membrane localization of Vps34 and its activity. Vps15 also engages with other key membrane proteins, such as the Rab5 GTPase to coordinate Vps34 activity at endosomes. Vps34 predominantly regulates membrane trafficking, with central roles in endosomal protein sorting, endosome–lysosome maturation, autophagosome formation, autophagy flux and cytokinesis.

特异性

PI3 Kinase Class 3 Rabbit Recombinant mAb detects endogenous level of total PI3 Kinase Class 3.

背景

PI3Ks are spatially regulated and multifunctional, and together, act at nearly all membranes in the cell to regulate a wide range of signaling, membrane trafficking and metabolic processes. There are three different PI3K classes (I, II and III) and different isoforms within each class. All three PI3K classes (I, II and III) phosphorylate the 3′-position hydroxyl of the D-myo-inositol head group to generate specific phosphoinositide forms. The PI3K family appears restricted to eukaryotes, and only the class III PI3K is conserved from yeast to human. Unlike class I and II PI 3-kinases, class III PI3K can phosphorylate only PtdIns and is thus a PtdIns 3-kinase. The single class 3 PI3K, that is Vps34, binds to the adaptor protein Vps15 which is N-terminally myristoylated and regulates the intracellular membrane localization of Vps34 and its activity. Vps15 also engages with other key membrane proteins, such as the Rab5 GTPase to coordinate Vps34 activity at endosomes. Vps34 predominantly regulates membrane trafficking, with central roles in endosomal protein sorting, endosome–lysosome maturation, autophagosome formation, autophagy flux and cytokinesis.

使用信息

抗体应用

WB, IHC,ELISA,ELISA

稀释比例

WB	IHC
1:500	1:50

反应性

Human Mouse Rat

MW (kDa)

100kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

Application Data

WB

Validated by Selleck

IHC

Validated by Selleck