PI3 Kinase p85 beta Rabbit Recombinant mAb Datasheet

生物描述

特异性	PI3 Kinase p85 beta Rabbit Recombinant mAb detects endogenous level of total PI3 Kinase p85 beta.
背景	The phosphoinositide 3-kinase (PI3K) signaling pathway critically regulates cell growth and cell survival. PI3K is a lipid kinase composed of a heterodimer made up of p85 regulatory subunit(s) and p110 catalytic subunit(s). The p85 regulatory subunit is a phosphoprotein substrate for a variety of cytoplasmic and receptor kinases. Class 1A PI3K is a dimer consisting of a p85 regulatory subunit and a p110 catalytic subunit. The p85 regulatory subunit is essential for the stability of the p110 catalytic subunit, and under resting state p85 represses the catalytic activity of p110. The two major regulatory subunits are p85α and p85β. p85β induces cellular transformation of primary fibroblasts, increased cell proliferation and elevated PI3K signaling. The oncogenic activity of p85β depends on active PI3K and TOR signaling and is mediated by two PI3K catalytic isoforms, p110α and p110β. Elevated expression of p85β stimulates PI3K signaling, enhances cellular invasiveness and is linked to tumor progression. It plays a role in the formation of cellular invadopodia and affects actin polymerization and rearrangement.

特异性

PI3 Kinase p85 beta Rabbit Recombinant mAb detects endogenous level of total PI3 Kinase p85 beta.

背景

The phosphoinositide 3-kinase (PI3K) signaling pathway critically regulates cell growth and cell survival. PI3K is a lipid kinase composed of a heterodimer made up of p85 regulatory subunit(s) and p110 catalytic subunit(s). The p85 regulatory subunit is a phosphoprotein substrate for a variety of cytoplasmic and receptor kinases. Class 1A PI3K is a dimer consisting of a p85 regulatory subunit and a p110 catalytic subunit. The p85 regulatory subunit is essential for the stability of the p110 catalytic subunit, and under resting state p85 represses the catalytic activity of p110. The two major regulatory subunits are p85α and p85β. p85β induces cellular transformation of primary fibroblasts, increased cell proliferation and elevated PI3K signaling. The oncogenic activity of p85β depends on active PI3K and TOR signaling and is mediated by two PI3K catalytic isoforms, p110α and p110β. Elevated expression of p85β stimulates PI3K signaling, enhances cellular invasiveness and is linked to tumor progression. It plays a role in the formation of cellular invadopodia and affects actin polymerization and rearrangement.

使用信息

抗体应用

WB, IHC, IF,ELISA,ELISA

稀释比例

WB	IHC	IF
1:1000	1:50	1:50

反应性

Human Rat

MW (kDa)

82kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

[4] Ito Y, et al. Proc Natl Acad Sci U S A. 2014, 111(47):16826-9.

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Application Data

WB

Validated by Selleck

IHC

Validated by Selleck

IF

Validated by Selleck