PKC theta Rabbit Recombinant mAb Datasheet

生物描述

特异性	PKC theta Rabbit Recombinant mAb detects endogenous level of total PKC theta.
背景	PKC includes a large family of homologous serine/threonine protein kinases that is widely conserved in eukaryotes. In mammals, there are 12 isoforms that are identified and subdivided into three groups based on their divergent regulatory domains and their second messenger requirements for activation: the conventional PKCs (cPKCs: α, βI, βII, and γ); novel PKCs (nPKCs: δ, ϵ, η, and θ); and atypical PKCs (aPKCs: ι/λ) and ζ). The intrinsic PKC-θ kinase activity is regulated through an allosteric mechanism, which leads to the change in PKC-θ conformation between “closed/inactive” and “open/active” state. Upon the initial receptor stimulation, PKC-θ is recruited to plasma membrane via membrane-resident DAG binding to its C1 domain. This triggers the conformational change of PKC-θ from “close” to “open” state, which allows its activation loop in the kinase domain to be accessible for the phosphorylation by germinal center kinase-like kinase (GLK, also known as MAP4K3). It plays an integral role in activating a range of signaling cascades that ultimately results in a transcriptional network in T cells.

特异性

PKC theta Rabbit Recombinant mAb detects endogenous level of total PKC theta.

背景

PKC includes a large family of homologous serine/threonine protein kinases that is widely conserved in eukaryotes. In mammals, there are 12 isoforms that are identified and subdivided into three groups based on their divergent regulatory domains and their second messenger requirements for activation: the conventional PKCs (cPKCs: α, βI, βII, and γ); novel PKCs (nPKCs: δ, ϵ, η, and θ); and atypical PKCs (aPKCs: ι/λ) and ζ). The intrinsic PKC-θ kinase activity is regulated through an allosteric mechanism, which leads to the change in PKC-θ conformation between “closed/inactive” and “open/active” state. Upon the initial receptor stimulation, PKC-θ is recruited to plasma membrane via membrane-resident DAG binding to its C1 domain. This triggers the conformational change of PKC-θ from “close” to “open” state, which allows its activation loop in the kinase domain to be accessible for the phosphorylation by germinal center kinase-like kinase (GLK, also known as MAP4K3). It plays an integral role in activating a range of signaling cascades that ultimately results in a transcriptional network in T cells.

使用信息

抗体应用

WB,ELISA,ELISA

稀释比例

WB

1:1000

反应性

Human Mouse Rat

MW (kDa)

82kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

Application Data

WB

Validated by Selleck