PKM2 Rabbit Recombinant mAb Datasheet

生物描述

特异性	PKM2 Rabbit Recombinant mAb detects endogenous level of total PKM2.
背景	Pyruvate kinase (PK) is a rate-limiting glycolytic enzyme that catalyzes the irreversible transphosphorylation between phosphoenolpyruvate (PEP) and adenosine diphosphate, which produces pyruvate and ATP. In mammals, the PK family consists of four isoforms: liver-type PK (PKL); red blood cell PK (PKR); and PK muscle isozyme M1 and M2 (PKM1 and PKM2, respectively). These isoforms are encoded by two genes, PK, liver and red blood cell (PKLR) and PK, muscle (PKM). PKM1 and PKM2 are produced by alternative splicing of the primary RNA transcript of the PKM gene. The non-allosteric isoform PKM1 is constitutively active, and expressed in terminally differentiated tissues, including the muscle and brain, which require a large supply of ATP. By contrast, PKM2 is expressed in tissues with anabolic functions, including proliferating cells and cancer cells, and is subject to complex allosteric regulation. Pyruvate kinase muscle isozyme M2 (PKM2) is a limiting glycolytic enzyme that catalyzes the final step in glycolysis, which is key in tumor metabolism and growth. This reaction is a committed step that leads to glycolysis or oxidative phosphorylation of pyruvate.

特异性

PKM2 Rabbit Recombinant mAb detects endogenous level of total PKM2.

背景

Pyruvate kinase (PK) is a rate-limiting glycolytic enzyme that catalyzes the irreversible transphosphorylation between phosphoenolpyruvate (PEP) and adenosine diphosphate, which produces pyruvate and ATP. In mammals, the PK family consists of four isoforms: liver-type PK (PKL); red blood cell PK (PKR); and PK muscle isozyme M1 and M2 (PKM1 and PKM2, respectively). These isoforms are encoded by two genes, PK, liver and red blood cell (PKLR) and PK, muscle (PKM). PKM1 and PKM2 are produced by alternative splicing of the primary RNA transcript of the PKM gene. The non-allosteric isoform PKM1 is constitutively active, and expressed in terminally differentiated tissues, including the muscle and brain, which require a large supply of ATP. By contrast, PKM2 is expressed in tissues with anabolic functions, including proliferating cells and cancer cells, and is subject to complex allosteric regulation. Pyruvate kinase muscle isozyme M2 (PKM2) is a limiting glycolytic enzyme that catalyzes the final step in glycolysis, which is key in tumor metabolism and growth. This reaction is a committed step that leads to glycolysis or oxidative phosphorylation of pyruvate.

使用信息

抗体应用

WB, IHC,ELISA,ELISA

稀释比例

WB	IHC
1:1000	1:50

反应性

Human Mouse Rat

MW (kDa)

58kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

[1] Dong G, et al. Oncol Lett. 2016, 11(3):1980-1986.

Application Data

WB

Validated by Selleck

IHC

Validated by Selleck