PP2A alpha/beta Rabbit Recombinant mAb Datasheet

生物描述

特异性	PP2A alpha/beta Rabbit Recombinant mAb detects endogenous level of total PP2A alpha/beta.
背景	Protein Phosphatase 2A (PP2A) is an important and ubiquitously expressed serine threonine phosphatase and regulates the function by dephosphorylating many critical cellular molecules like Akt, p53, c-Myc and β-catenin. It plays a critical role in cellular processes, such as cell proliferation, signal transduction and apoptosis. Structurally, it is multifarious as it is composed of catalytic, scaffold and regulatory subunits. The regulatory subunit is the most diverse with temporal and spatial specificity. PP2A is structurally complex and exists in two different forms: dimeric form (PP2AD) and a trimeric form (PP2AT). The dimeric form is known as the core enzyme and is composed of the catalytic and scaffold subunit, while the trimeric form is an active holoenzyme complex which consists of three subunits: catalytic (PP2Ac), scaffold (PP2AA) and regulatory subunits (PP2AB). PP2A catalytic subunit (PP2Ac) is globular in structure, ubiquitously expressed in almost every tissue, and it is most abundant in the heart and brain. PP2Ac exists in two isoforms Cα and Cβ. The isoform Cα is predominantly expressed in the plasma membrane and Cβ in the cytoplasm and nucleus. Cα is expressed in higher abundance than Cβ due to its strong promoter activity as well as due to differences in the rate of mRNA turnover.

特异性

PP2A alpha/beta Rabbit Recombinant mAb detects endogenous level of total PP2A alpha/beta.

背景

Protein Phosphatase 2A (PP2A) is an important and ubiquitously expressed serine threonine phosphatase and regulates the function by dephosphorylating many critical cellular molecules like Akt, p53, c-Myc and β-catenin. It plays a critical role in cellular processes, such as cell proliferation, signal transduction and apoptosis. Structurally, it is multifarious as it is composed of catalytic, scaffold and regulatory subunits. The regulatory subunit is the most diverse with temporal and spatial specificity. PP2A is structurally complex and exists in two different forms: dimeric form (PP2AD) and a trimeric form (PP2AT). The dimeric form is known as the core enzyme and is composed of the catalytic and scaffold subunit, while the trimeric form is an active holoenzyme complex which consists of three subunits: catalytic (PP2Ac), scaffold (PP2AA) and regulatory subunits (PP2AB). PP2A catalytic subunit (PP2Ac) is globular in structure, ubiquitously expressed in almost every tissue, and it is most abundant in the heart and brain. PP2Ac exists in two isoforms Cα and Cβ. The isoform Cα is predominantly expressed in the plasma membrane and Cβ in the cytoplasm and nucleus. Cα is expressed in higher abundance than Cβ due to its strong promoter activity as well as due to differences in the rate of mRNA turnover.

使用信息

抗体应用

WB, IHC,ELISA,ELISA

稀释比例

WB	IHC
1:1000	1:50

反应性

Human Mouse Rat

MW (kDa)

35kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

[1] Seshacharyulu P, et al. Cancer Lett. 2013, 335(1):9-18.

Application Data

WB

Validated by Selleck

IHC

Validated by Selleck