PTBP2 Rabbit Recombinant mAb Datasheet

生物描述

特异性	PTBP2 Rabbit Recombinant mAb detects endogenous level of total PTBP2.
背景	PTB (polypyrimidine-tract-binding protein) is a ubiquitous RNA-binding protein. It was originally identified as a protein with a role in splicing but it is now known to function in a large number of diverse cellular processes including polyadenylation, mRNA stability and translation initiation. It is able to shuttle between the nucleus and the cytoplasm, a process that is tightly regulated since the diverse cellular functions which require PTB are dependent on its location (and on its associations with other factors). Different isoforms of PTB are the result of alternative splicing. They are expressed at different levels according to cell type and, despite their similarity, have been shown to have different activities in splicing and on IRES activity. PTBP1 and PTBP2 have distinct expression patterns. PTBP1 is widely expressed but is absent from neurons and muscle cells. PTBP2 is expressed in neurons, testis, and certain other cells. PTBP1 is expressed in neuronal progenitor cells but is replaced by PTBP2 during early neuronal differentiation. The switch from PTBP1 to PTBP2 alters the splicing of exons that are more sensitive to PTBP1, thus reprogramming neuronal splicing. The expression of PTBP2 is later reduced, leading to a second switch in the splicing program as neurons mature.

特异性

PTBP2 Rabbit Recombinant mAb detects endogenous level of total PTBP2.

背景

PTB (polypyrimidine-tract-binding protein) is a ubiquitous RNA-binding protein. It was originally identified as a protein with a role in splicing but it is now known to function in a large number of diverse cellular processes including polyadenylation, mRNA stability and translation initiation. It is able to shuttle between the nucleus and the cytoplasm, a process that is tightly regulated since the diverse cellular functions which require PTB are dependent on its location (and on its associations with other factors). Different isoforms of PTB are the result of alternative splicing. They are expressed at different levels according to cell type and, despite their similarity, have been shown to have different activities in splicing and on IRES activity. PTBP1 and PTBP2 have distinct expression patterns. PTBP1 is widely expressed but is absent from neurons and muscle cells. PTBP2 is expressed in neurons, testis, and certain other cells. PTBP1 is expressed in neuronal progenitor cells but is replaced by PTBP2 during early neuronal differentiation. The switch from PTBP1 to PTBP2 alters the splicing of exons that are more sensitive to PTBP1, thus reprogramming neuronal splicing. The expression of PTBP2 is later reduced, leading to a second switch in the splicing program as neurons mature.

使用信息

抗体应用

WB, IF,ELISA,ELISA

稀释比例

WB	IF
1:1000	1:50

反应性

Human Mouse Rat

MW (kDa)

57kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

Application Data

WB

Validated by Selleck

IF

Validated by Selleck