Pyruvate Dehydrogenase E1 beta subunit Rabbit Recombinant mAb

生物描述

特异性

Pyruvate Dehydrogenase E1 Rabbit Recombinant mAb detects endogenous level of total Pyruvate Dehydrogenase E1.

背景

The pyruvate dehydrogenase complex (PDHC) represents a member of a multienzyme complex family that also comprises the 2-oxoglutarate dehydrogenase complex (OGDHC) and the branched-chain 2-oxoacid dehydrogenase complex (BCOADHC). These enzymes catalyze the oxidative decarboxylation of pyruvate, 2-oxoglutarate, and the 2-oxo acids of the branched-chain amino acids l-leucine, l-valine, and l-isoleucine, respectively. In general, the multienzyme complexes are composed of multiple copies of three different enzymes, a thiamine pyrophosphate (TPP) containing 2-oxoacid decarboxylase (E1), a lipoic acid-containing dihydrolipoamide acyltransferase (E2), and the flavin-containing lipoamide dehydrogenase (LPD). The E1 enzyme catalyzes the irreversible, TPP-dependent oxidative decarboxylation of the 2-oxoacid, followed by the acylation of the lipoyl prosthetic group covalently attached to the E2 chain.

使用信息

抗体应用	WB, IHC, IF,ELISA,ELISA
稀释比例	WB IHC IF 1:1000 1:50 1:50
反应性	Human Mouse Rat
MW (kDa)	35kDa
抗体类型	Rabbit
储存液配方	10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.
储存条件 (自收到货起)	Store at –20°C.

文献参考

[1] Schreiner ME, et al. J Bacteriol. 2005, 187(17):6005-18.

Application Data

WB

IHC

IF