Datasheet

生物描述

特异性	Ran Rabbit Recombinant mAb detects endogenous levels of total Ran.
背景	Ran is a small GTP-binding protein of the Ras superfamily regulating fundamental cellular processes: nucleo-cytoplasmic transport, nuclear envelope formation and mitotic spindle assembly. Ran is a typical G-protein in that it cycles between a GDP-bound and GTP-bound state. Ran is a typical G-protein in that it cycles between a GDP-bound and GTP-bound state. In interphase cells, about 90% of cellular Ran is nuclear, and only a minor proportion is cytosolic. In the nucleus, Ran•GTP binds to exportins such as CRM1 (Chromosome region maintenance 1) to transport cargo proteins containing a nuclear export signal (NES) into the cytosol. Ran•GTP, furthermore, binds to Importin-β•cargo complexes to release the cargo in the nucleus. In the cytosol, the Importin•Ran•GTP complexes, as well as the ternary exportin•Ran•GTP-cargo complexes, dissociate on binding of RanBP1 and subsequent GTP hydrolysis catalyzed by RanGAP. The Ran transport cycle closes by translocation of Ran•GDP to the nucleus by the nuclear transport factor 2 (NTF2). Many of these Ran interactions also play important roles in mitotic spindle assembly and nuclear envelope formation. Ran could be lysine acetylated at five distinct sites in human (K37, K60, K71, K99, and K159). Acetylation of Ran at K37, K99, and K159 increases binding toward Importin-β predominantly due to a strong decrease in the complex dissociation rates and also increases the binding of Spn1 to a preformed CRM1•Ran•GppNHp complex. In addition, Ran plays important roles in diverse cellular processes such as nucleo-cytoplasmic transport, mitotic spindle formation, and nuclear envelope assembly.

特异性

Ran Rabbit Recombinant mAb detects endogenous levels of total Ran.

背景

Ran is a small GTP-binding protein of the Ras superfamily regulating fundamental cellular processes: nucleo-cytoplasmic transport, nuclear envelope formation and mitotic spindle assembly. Ran is a typical G-protein in that it cycles between a GDP-bound and GTP-bound state. Ran is a typical G-protein in that it cycles between a GDP-bound and GTP-bound state. In interphase cells, about 90% of cellular Ran is nuclear, and only a minor proportion is cytosolic. In the nucleus, Ran•GTP binds to exportins such as CRM1 (Chromosome region maintenance 1) to transport cargo proteins containing a nuclear export signal (NES) into the cytosol. Ran•GTP, furthermore, binds to Importin-β•cargo complexes to release the cargo in the nucleus. In the cytosol, the Importin•Ran•GTP complexes, as well as the ternary exportin•Ran•GTP-cargo complexes, dissociate on binding of RanBP1 and subsequent GTP hydrolysis catalyzed by RanGAP. The Ran transport cycle closes by translocation of Ran•GDP to the nucleus by the nuclear transport factor 2 (NTF2). Many of these Ran interactions also play important roles in mitotic spindle assembly and nuclear envelope formation. Ran could be lysine acetylated at five distinct sites in human (K37, K60, K71, K99, and K159). Acetylation of Ran at K37, K99, and K159 increases binding toward Importin-β predominantly due to a strong decrease in the complex dissociation rates and also increases the binding of Spn1 to a preformed CRM1•Ran•GppNHp complex. In addition, Ran plays important roles in diverse cellular processes such as nucleo-cytoplasmic transport, mitotic spindle formation, and nuclear envelope assembly.

使用信息

抗体应用

wb,ELISA,ELISA

稀释比例

WB

1:1000

反应性

Human Mouse Rat

MW (kDa)

24kDa

抗体类型

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

Application Data

WB

Validated by Selleck