Datasheet

生物描述

特异性	Raptor Rabbit Recombinant mAb detects endogenous levels of total Raptor.
背景	Raptor is a mTOR binding protein that also binds 4EBP1 and p70α through which mTOR could control cell growth. The binding of raptor to mTOR is necessary for the mTOR-catalyzed phosphorylation of 4EBP1 in vitro, and it strongly enhances the mTOR kinase activity toward p70α. Raptor plays a pivotal role as a scaffold protein in the mTOR-signaling pathway that appears to be phylogenetically conserved. Raptor-mTOR complex positively regulates cell growth and its inhibition causes a large decrease in cell size. The raptor branch of the mTOR pathway modulates a stunning number of major processes, including mRNA translation, ribosome biogenesis, nutrient metabolism and autophagy. Raptor-mTOR activates S6K1, and likely the related S6K2, by phosphorylating it within the hydrophobic motif conserved in the AGC family of kinases. The raptor-mTOR pathway regulates cell growth (accumulation of cell mass) through S6K1 and 4E-BP1 as well as unknown effectors. It responds to nutrients and growth factors in part through the upstream regulators TSC1/2 and rheb.

特异性

Raptor Rabbit Recombinant mAb detects endogenous levels of total Raptor.

背景

Raptor is a mTOR binding protein that also binds 4EBP1 and p70α through which mTOR could control cell growth. The binding of raptor to mTOR is necessary for the mTOR-catalyzed phosphorylation of 4EBP1 in vitro, and it strongly enhances the mTOR kinase activity toward p70α. Raptor plays a pivotal role as a scaffold protein in the mTOR-signaling pathway that appears to be phylogenetically conserved. Raptor-mTOR complex positively regulates cell growth and its inhibition causes a large decrease in cell size. The raptor branch of the mTOR pathway modulates a stunning number of major processes, including mRNA translation, ribosome biogenesis, nutrient metabolism and autophagy. Raptor-mTOR activates S6K1, and likely the related S6K2, by phosphorylating it within the hydrophobic motif conserved in the AGC family of kinases. The raptor-mTOR pathway regulates cell growth (accumulation of cell mass) through S6K1 and 4E-BP1 as well as unknown effectors. It responds to nutrients and growth factors in part through the upstream regulators TSC1/2 and rheb.

使用信息

抗体应用

wb,ELISA,ELISA

稀释比例

WB

1:500

反应性

Human Mouse Rat

MW (kDa)

149kDa

抗体类型

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

Application Data

WB

Validated by Selleck