Recombinant Human Activin A Protein, BSA (HEK293) Datasheet

生物描述

Accession

P08476, NP_002183.1

别名

EDF; FRP; INHBA; Activin A

背景

激活素是二聚体生长与分化因子，属于结构相关的信号蛋白——转化生长因子-β（TGF-β）超家族。激活素通过受体丝氨酸激酶组成的异源复合体传递信号，该复合体至少包含两种I型受体（I型和IB型）和两种II型受体（II型和IIB型）。这些受体均为跨膜蛋白，由富含半胱氨酸的配体结合胞外域、跨膜域以及具有预测丝氨酸/苏氨酸特异性的胞内域构成。I型受体对信号传导至关重要；II型受体则负责配体结合及调控I型受体的表达。配体结合后，I型与II型受体形成稳定复合物，导致II型受体磷酸化I型受体。激活素A的突变与进行性骨化性纤维发育不良相关。

使用信息

物种

Human

标签

No tag

来源

HEK293-drived Human Activin A Protein AA Gly311-Ser426.

预测分子量

12.97 kDa

实际分子量

14-15 kDa, reducing conditions

纯度

>95% as determined by Bis-Tris PAGE.

内毒素

< 0.1 EU/ug by the LAL method

制剂

Lyophilized from a 0.22 μm filtered solution in PBS with BSA as a carrier protein. Normally 8% trehalose is added as protectant before lyophilization.

复溶方法

开盖前先10,000-12,000 rpm 离心 30 s；向冻干粉中加入无菌PBS，用枪头轻轻吹打混匀，重悬浓度不低于 100 μg/mL；复溶之后分装放-80℃保存，避免反复冻融。

储存条件 (自收到货起)

For long-term storage, keep the product lyophilized at -20°C or below. Please avoid repeated freeze-thaw cycles. Stability: Lyophilized: 12 months (-20°C to -80°C) Reconstituted: 3 months at -80°C under sterile conditions.

Data

: Recombinant Human Activin A Protein, BSA (HEK293)on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.