Recombinant Human CD2 / SRBC Protein, CF (HEK293)

目录号：C0452

打印

Recombinant Human CD2 / SRBC Protein, CF (HEK293) is expressed from HEK293 cells with an His tag at the C-terminus.

生物描述

Accession

P06729

别名

CD2, SRBC, LFA-2, T11

背景

T细胞表面抗原 CD2，又称红细胞受体、LFA‑2、LFA‑3 受体、玫瑰花结受体、T 细胞表面抗原 T11/Leu‑5 以及 SRBC，是表达于 T 细胞和自然杀伤（NK）细胞表面的单次跨膜 I 型膜蛋白。CD2 属于免疫球蛋白超家族。CD2/SRBC 包含 1 个 Ig 样 C2 型结构域和 1 个 Ig 样 V 型结构域。CD2/SRBC 可与其他黏附分子发生相互作用：在人体内为淋巴细胞功能相关抗原‑3（LFA‑3 / CD58），在啮齿类动物中为 CD48，二者均表达于相邻细胞表面。除黏附功能外，CD2 还可作为 T 细胞与 NK 细胞的共刺激分子。CD2 是 T 细胞和 NK 细胞的特异性标志物，因此可用于免疫组化，在组织切片中检出该类细胞群。

使用信息

物种

Human

标签

His tag at the C-terminus

来源

HEK293-drived Human CD2 / SRBC Protein AA Lys 25 - Asp 209.

预测分子量

22.3 kDa

实际分子量

33-40 kDa, reducing conditions

纯度

>95% as determined by Bis-Tris PAGE.

内毒素

< 0.1 EU/ug by the LAL method

制剂

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

复溶方法

开盖前先10,000-12,000 rpm 离心 30 s；向冻干粉中加入无菌PBS，用枪头轻轻吹打混匀，重悬浓度不低于 100 μg/mL；复溶之后分装放-80℃保存，避免反复冻融，如要长期储存，建议添加保护剂（如0.1% BSA、5% HSA、10% FBS等）

储存条件 (自收到货起)

For long-term storage, keep the product lyophilized at -20°C or below. Please avoid repeated freeze-thaw cycles. Stability: Lyophilized: 12 months (-20°C to -80°C) Reconstituted: 3 months at -80°C under sterile conditions.

Data

: Recombinant Human CD2 / SRBC Protein, CF (HEK293)on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.