Recombinant Human HMGB1 Protein, CF (HEK293)

目录号：C0148

打印

Recombinant Human HMGB1 Protein is expressed from HEK293 with His tag at the C-terminus.

生物描述

Accession

P09429-1

别名

HMGB1,HMG1,HMG3,SBP-1

背景

高迁移率族蛋白 B1 (HMGB1) 也称为高迁移率族蛋白 1 (HMG-1) 和双性蛋白，是 HMGB 家族的成员，该家族由三个成员组成：HMGB1、HMGB2 和 HMGB3。HMGB1 是一种非组蛋白结构染色体蛋白，普遍存在于所有脊椎动物细胞核中，并无序列特异性结合双链 DNA。炎症和损伤的机制是与 TLR4 结合，后者介导 HMGB1 依赖性巨噬细胞因子释放的激活。这使 HMGB1 处于无菌性和感染性炎症反应的交叉点。HMGB1 已被研究作为 DNA 疫苗佐剂和癌症治疗的靶点。

使用信息

物种

Human

标签

His tag at the C-terminus.

来源

HEK293-drived human HMGB1 protein AA Met 1 - Glu 215.

预测分子量

27 kDa

实际分子量

32-35 kDa , reducing conditions

纯度

>95% as determined by Bis-Tris PAGE.

内毒素

< 0.1 EU/ug by the LAL method

制剂

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

复溶方法

开盖前先10,000-12,000 rpm 离心 30 s；向冻干粉中加入无菌PBS，用枪头轻轻吹打混匀，重悬浓度低于 100 μg/mL；复溶之后分装放-80℃保存，避免反复冻融，如要长期储存，建议添加保护剂（如0.1% BSA、5% HSA、10% FBS等）

储存条件 (自收到货起)

For long-term storage, keep the product lyophilized at -20°C or below. Please avoid repeated freeze-thaw cycles. Stability: Lyophilized: 12 months (-20°C to -80°C) Reconstituted: 3 months at -80°C under sterile conditions

Data

: Recombinant Human HMGB1 Protein, CF (HEK293)on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.