Rho A/B/C Rabbit Recombinant mAb

生物描述

特异性

Rho A/B/C Rabbit Recombinant mAb detects endogenous level of total Rho A/B/C.

背景

Rho proteins have been involved in various biological processes such as control of signalling pathways, cellular proliferation and apoptosis. The Rho family of small guanine nucleotide-binding proteins comprises several members: RhoA, RhoB, RhoC, RhoG, RhoE, Rac1, Rac2, Cdc42Hs, and TC10. Rho GTPases, RhoA, RhoB, and RhoC share 85% amino acid sequence identity. Despite their similarity, some regulators and effectors show preferential interaction with RhoA, B, or C, and the three proteins show differences in function in cells. RhoA plays a key role in the regulation of actomyosin contractility. RhoB, which is localized primarily on endosomes, has been shown to regulate cytokine trafficking and cell survival, while RhoC may be more important in cell locomotion.

使用信息

抗体应用	WB, IHC, IF,ELISA,ELISA
稀释比例	WB IHC IF 1:1000 1:50 1:50
反应性	Human Mouse Rat
MW (kDa)	22kDa
抗体类型	Rabbit
储存液配方	10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.
储存条件 (自收到货起)	Store at –20°C.

文献参考

[1] Javier Gómez, et al. Immunology and Cell Biology. 1998, 76: 125-134. [2] Wheeler AP, et al. Exp Cell Res. 2004, 301(1):43-9.

Application Data

WB

IHC

IF