Datasheet

生物描述

特异性	RhoGDI Rabbit Recombinant mAb detects endogenous levels of total RhoGDI
背景	RhoGDI (Rho GDP-dissociation inhibitor) is a down-regulator of Rho family GTPases typified by its ability to prevent nucleotide exchange and membrane association. RhoGDI constitutes a family with three mammalian members: RhoGDIα, the ubiquitously expressed archetypal member of the family; Ly/D4-GDI or RhoGDIβ, which has haematopoietic tissue-specific expression, particularly in B- and T-lymphocytes; and RhoGDI-3 or -γ, which is membrane-anchored through an amphipathic helix and is preferentially expressed in brain, pancreas, lung, kidney and testis. GDI acts as a negative regulator of Rho-family signalling, blocking GEF-mediated exchange and maintaining the GTPase in an inactive GDP-bound state. RhoGDI is also able to bind with high affinity to the GTP-bound form of the Rho family member Cdc42 to act as a GTPase inhibitory protein (GIP), blocking both intrinsic and GAP-catalyzed GTP-hydrolysis and thus maintaining Cdc42 in a GTP-bound conformation. It solubilizes Cdc42 from cellular membranes. RhoGDI binds only to the prenylated form of Rho proteins, and GDI binding sequesters the lipid moiety from the membrane, creating a soluble cytosolic GDI/GTPase complex. Appropriate localization of Rho proteins is critical for their biological function, and regulation of this localization by RhoGDI is likely to play an important role in signalling through Rho GTPases. RhoGDI could control the partitioning between the cytosol and membrane compartments and may facilitate the targeting of GTPases to appropriate signalling sites.

特异性

RhoGDI Rabbit Recombinant mAb detects endogenous levels of total RhoGDI

背景

RhoGDI (Rho GDP-dissociation inhibitor) is a down-regulator of Rho family GTPases typified by its ability to prevent nucleotide exchange and membrane association. RhoGDI constitutes a family with three mammalian members: RhoGDIα, the ubiquitously expressed archetypal member of the family; Ly/D4-GDI or RhoGDIβ, which has haematopoietic tissue-specific expression, particularly in B- and T-lymphocytes; and RhoGDI-3 or -γ, which is membrane-anchored through an amphipathic helix and is preferentially expressed in brain, pancreas, lung, kidney and testis. GDI acts as a negative regulator of Rho-family signalling, blocking GEF-mediated exchange and maintaining the GTPase in an inactive GDP-bound state. RhoGDI is also able to bind with high affinity to the GTP-bound form of the Rho family member Cdc42 to act as a GTPase inhibitory protein (GIP), blocking both intrinsic and GAP-catalyzed GTP-hydrolysis and thus maintaining Cdc42 in a GTP-bound conformation. It solubilizes Cdc42 from cellular membranes. RhoGDI binds only to the prenylated form of Rho proteins, and GDI binding sequesters the lipid moiety from the membrane, creating a soluble cytosolic GDI/GTPase complex. Appropriate localization of Rho proteins is critical for their biological function, and regulation of this localization by RhoGDI is likely to play an important role in signalling through Rho GTPases. RhoGDI could control the partitioning between the cytosol and membrane compartments and may facilitate the targeting of GTPases to appropriate signalling sites.

使用信息

抗体应用

WB, IHC,ELISA,ELISA

稀释比例

WB	IHC
1:1000	1:50

反应性

Human Mouse Rat

MW (kDa)

26kDa

抗体类型

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

Application Data

WB

Validated by Selleck

IHC

Validated by Selleck