全国免费电话:400-668-6834 -- 仅限中国地区 -- |
在线订购:QQ: 4006686834 电话订购:021-68591985 邮件订购:info@selleck.cn 我们也提供销售人员上门服务 |
技术支持电话:400-168-6834 我们将于一个工作日与您联系 |
特异性 | ROCK1 Rabbit Recombinant mAb detects endogenous level of ROCK1. |
---|---|
背景 | Rho-associated kinases ROCK1 and ROCK2 are serine/threonine kinases that are downstream targets of the small GTPases RhoA, RhoB, and RhoC. ROCKs are involved in diverse cellular activities including actin cytoskeleton organization, cell adhesion and motility, proliferation and apoptosis, remodeling of the extracellular matrix and smooth muscle cell contraction. Moreover, depending on their subcellular localization, activation, and other environmental factors, ROCK signaling can have different effects on cellular function. For ROCK2 a cytosolic and nuclear localization, association with the centrosome, and co-localization with actin and vimentin filaments in different cell types were reported, as well as its localization at the intercalated disk and Z-disk of striated muscle cells. ROCK2 accumulates within the cleavage furrow during late mitosis and may has a role in the formation of the contractile ring during cytokinesis. In contrast to ROCK2, there is less information on the subcellular localization of ROCK1. However, it has been reported to have a cytosolic localization, as well as association with centrosomes, the plasma membrane, cell–cell contacts, cell adhesion sites, and vesicles. The expression of ROCK1 and ROCK2 also varies between different tissues: higher abundance of ROCK2 in heart and brain and a more prominent expression of ROCK1 in blood cells and the thymus. |
抗体应用 | WB,ELISA,ELISA | ||
---|---|---|---|
稀释比例 |
|
||
反应性 | Human Mouse Rat | ||
MW (kDa) | 158kDa | ||
抗体类型 | Rabbit | ||
储存液配方 | 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. | ||
储存条件 (自收到货起) |
Store at –20°C. |
WB
Validated by Selleck