ROCK2 Rabbit Recombinant mAb Datasheet

生物描述

特异性	ROCK2 Rabbit Recombinant mAb detects endogenous level of ROCK2.
背景	Rho-associated kinases ROCK1 and ROCK2 are serine/threonine kinases that are downstream targets of the small GTPases RhoA, RhoB, and RhoC. ROCKs are involved in diverse cellular activities including actin cytoskeleton organization, cell adhesion and motility, proliferation and apoptosis, remodeling of the extracellular matrix and smooth muscle cell contraction. Moreover, depending on their subcellular localization, activation, and other environmental factors, ROCK signaling can have different effects on cellular function. For ROCK2 a cytosolic and nuclear localization, association with the centrosome, and co-localization with actin and vimentin filaments in different cell types were reported, as well as its localization at the intercalated disk and Z-disk of striated muscle cells. ROCK2 accumulates within the cleavage furrow during late mitosis and may has a role in the formation of the contractile ring during cytokinesis. In contrast to ROCK2, there is less information on the subcellular localization of ROCK1. However, it has been reported to have a cytosolic localization, as well as association with centrosomes, the plasma membrane, cell–cell contacts, cell adhesion sites, and vesicles. The expression of ROCK1 and ROCK2 also varies between different tissues: higher abundance of ROCK2 in heart and brain and a more prominent expression of ROCK1 in blood cells and the thymus.

特异性

ROCK2 Rabbit Recombinant mAb detects endogenous level of ROCK2.

背景

Rho-associated kinases ROCK1 and ROCK2 are serine/threonine kinases that are downstream targets of the small GTPases RhoA, RhoB, and RhoC. ROCKs are involved in diverse cellular activities including actin cytoskeleton organization, cell adhesion and motility, proliferation and apoptosis, remodeling of the extracellular matrix and smooth muscle cell contraction. Moreover, depending on their subcellular localization, activation, and other environmental factors, ROCK signaling can have different effects on cellular function. For ROCK2 a cytosolic and nuclear localization, association with the centrosome, and co-localization with actin and vimentin filaments in different cell types were reported, as well as its localization at the intercalated disk and Z-disk of striated muscle cells. ROCK2 accumulates within the cleavage furrow during late mitosis and may has a role in the formation of the contractile ring during cytokinesis. In contrast to ROCK2, there is less information on the subcellular localization of ROCK1. However, it has been reported to have a cytosolic localization, as well as association with centrosomes, the plasma membrane, cell–cell contacts, cell adhesion sites, and vesicles. The expression of ROCK1 and ROCK2 also varies between different tissues: higher abundance of ROCK2 in heart and brain and a more prominent expression of ROCK1 in blood cells and the thymus.

使用信息

抗体应用

WB,ELISA,ELISA

稀释比例

WB

1:1000

反应性

Human Mouse Rat

MW (kDa)

161kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

[1] Hartmann S, et al. Front Pharmacol. 2015, 6:276.

Application Data

WB

Validated by Selleck