Smad2 Rabbit Recombinant mAb

生物描述

特异性

Smad2 Rabbit Recombinant mAb detects endogenous levels of total Smad2.

背景

Transforming growth factor-b superfamily member signals are conveyed through cell-surface serine/threonine kinase receptors to the intracellular mediators known as Smads. Activation of Smads causes their translocation from the cytoplasm to the nucleus where they function to control gene expression. The Smad family of proteins can be divided into three functional groups: the receptor-activated Smads (R-Smads), common mediator Smads (Co-Smads), and the inhibitory Smads. The R-Smads are directly phosphorylated by the activated type I receptors on their C-terminal Ser-Ser-X-Ser (SSXS) motif and include Smad1, Smad2, Smad3, Smad5, and Smad8. Smad2 and Smad3 are phosphorylated in response to TGF-b and activin, whereas Smad1, Smad5, and Smad8 are phosphorylated in response to BMP.

使用信息

抗体应用	WB, IF,ELISA,ELISA
稀释比例	WB IF 1:1000 1:50
反应性	Human Rat
MW (kDa)	58kDa
抗体类型	Rabbit
储存液配方	10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.
储存条件 (自收到货起)	Store at –20°C.

文献参考

[1] Wrana JL, et al. Cytokine Growth Factor Rev. 2000, 11(1-2):5-13. [2] Brown KA, et al. J Cell Biochem. 2007, 101(1):9-33.

Application Data

WB

IF