SMAD5 Rabbit Recombinant mAb

生物描述

特异性

SMAD5 Rabbit Recombinant mAb detects endogenous level of total SMAD5.

背景

Transforming growth factor-β superfamily member signals are conveyed through cell-surface serine/threonine kinase receptors to the intracellular mediators known as Smads. Activation of Smads causes their translocation from the cytoplasm to the nucleus where they function to control gene expression. Only five of the mammalian Smads—Smad1, Smad2, Smad3, Smad5, and Smad8—act as substrates for the TGFβ family of receptors. Smads 1, 5, and 8 serve principally as substrates for the BMP and anti-Muellerian receptors, and Smads 2 and 3 for the TGFβ, activin, and Nodal receptors. Smad4, also referred to as Co-Smad, serves as a common partner for all RSmads. Smad6 and Smad7 are inhibitory Smads that serve as decoys interfering with Smad–receptor or Smad–Smad interactions. Smad5 is known to transduce intracellular signals from bone morphogenetic proteins (BMPs), which belong to the transforming growth factor-β (TGF-β) superfamily and are involved in the regulation of hematopoiesis.

使用信息

抗体应用	WB, IHC, IF,ELISA,ELISA
稀释比例	WB IHC IF 1:1000 1:50 1:50
反应性	Human Rat
MW (kDa)	52kDa
抗体类型	Rabbit
储存液配方	10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.
储存条件 (自收到货起)	Store at –20°C.

文献参考

[1] Joan Massagué, et al. Genes. 2005, 19: 2783-2810. [2] Wrana JL, et al. Cytokine Growth Factor Rev. 2000, 11(1-2):5-13. [3] Singbrant S, et al. Blood. 2006, 108(12):3707-12.

Application Data

WB

IHC

IF