Sonic Hedgehog Rabbit Recombinant mAb Datasheet

生物描述

特异性	Sonic Hedgehog Rabbit Recombinant mAb detects endogenous levels of total sonic hedgehog.
背景	The Hedgehog signalling pathway has diverse functions in animal development and tissue homeostasis. HH signalling regulates the survival and proliferation of tissue progenitor and stem populations. This function is linked to its role in tumour formation. There are three mammalian counterparts: sonic hedgehog (SHH), Indian hedgehog (IHH) and desert hedgehog (DHH). SHH activity reproduces the actions of the zone of polarizing activity in the limb bud and of the notochord and floor plate in the neural tube. Shh is the most broadly expressed mammalian Hh signaling molecule. During early vertebrate embryogenesis, Shh expressed in midline tissues such as the node, notochord, and floor plate controls patterning of the left–right and dorso-ventral axes of the embryo. Later in development, during organogenesis, Shh is expressed in and affects development of most epithelial tissues. The activity of Shh as a morphogen was initially thought to be due to concentration-dependent responses, but the duration of Shh signal seems also to critically affect cellular responses.

特异性

Sonic Hedgehog Rabbit Recombinant mAb detects endogenous levels of total sonic hedgehog.

背景

The Hedgehog signalling pathway has diverse functions in animal development and tissue homeostasis. HH signalling regulates the survival and proliferation of tissue progenitor and stem populations. This function is linked to its role in tumour formation. There are three mammalian counterparts: sonic hedgehog (SHH), Indian hedgehog (IHH) and desert hedgehog (DHH). SHH activity reproduces the actions of the zone of polarizing activity in the limb bud and of the notochord and floor plate in the neural tube. Shh is the most broadly expressed mammalian Hh signaling molecule. During early vertebrate embryogenesis, Shh expressed in midline tissues such as the node, notochord, and floor plate controls patterning of the left–right and dorso-ventral axes of the embryo. Later in development, during organogenesis, Shh is expressed in and affects development of most epithelial tissues. The activity of Shh as a morphogen was initially thought to be due to concentration-dependent responses, but the duration of Shh signal seems also to critically affect cellular responses.

使用信息

抗体应用

WB, IF,ELISA,ELISA

稀释比例

WB	IF
1:1000	1:50

反应性

Human

MW (kDa)

51kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

Application Data

WB

Validated by Selleck

IF

Validated by Selleck