Sumo2/3 Rabbit Recombinant mAb Datasheet

生物描述

特异性	Sumo2/3 Rabbit Recombinant mAb detects endogenous level of total Sumo2/3.
背景	SUMOylation is essential for cell cycle regulation in invertebrates; however, its functions during the mammalian cell cycle are largely uncharacterized. Mammals express three SUMO paralogues, SUMO-1, SUMO-2 and SUMO-3 (SUMO-2 and SUMO-3 are 96% identical and referred to as SUMO-2/3). SUMO-2/3 localize to centromeres and condensed chromosomes, whereas SUMO-1 localizes to the mitotic spindle and spindle midzone, indicating that SUMO paralogues regulate distinct mitotic processes in mammalian cells. All three SUMOs are covalently conjugated to other proteins through a common enzyme cascade involving the same E1 activating and E2 conjugating enzymes.

特异性

Sumo2/3 Rabbit Recombinant mAb detects endogenous level of total Sumo2/3.

背景

SUMOylation is essential for cell cycle regulation in invertebrates; however, its functions during the mammalian cell cycle are largely uncharacterized. Mammals express three SUMO paralogues, SUMO-1, SUMO-2 and SUMO-3 (SUMO-2 and SUMO-3 are 96% identical and referred to as SUMO-2/3). SUMO-2/3 localize to centromeres and condensed chromosomes, whereas SUMO-1 localizes to the mitotic spindle and spindle midzone, indicating that SUMO paralogues regulate distinct mitotic processes in mammalian cells. All three SUMOs are covalently conjugated to other proteins through a common enzyme cascade involving the same E1 activating and E2 conjugating enzymes.

使用信息

抗体应用

WB, IF,ELISA,ELISA

稀释比例

WB	IF
1:1000	1:50

反应性

Human Mouse Rat

MW (kDa)

16kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

[1] Zhang XD, et al. Mol Cell. 2008, 29(6):729-41.

Application Data

WB

Validated by Selleck

IF

Validated by Selleck