Thymidylate Synthase Rabbit Recombinant mAb Datasheet

生物描述

特异性	Thymidylate Synthase Rabbit Recombinant mAb detects endogenous level of total Thymidylate Synthase.
背景	Thymidylate synthase (TS) is a key enzyme in the synthesis of 2'-deoxythymidine-5'-monophosphate, an essential precursor for DNA biosynthesis. In addition to its role in catalysis and cellular metabolism, it also functions as an RNA binding protein. Thymidylate synthase binds with high affinity to its own mRNA resulting in translational repression. TS is involved in a complex pathway that is related to many other folate dependent enzymes; at least three pathways have been identified and completely or partially described. The first essential pathway is the TS cycle, which is devoted to the regeneration of the reduced and active folate form (methylentetrahydrofolate, mTHF), which is necessary for the reaction that leads to dTMP from dUMP. The second TS pathway is related to the feedback regulation of TS expression. The third and most recently identified pathway involving the TS enzyme is that of regulatory TS proteins or rTS.

特异性

Thymidylate Synthase Rabbit Recombinant mAb detects endogenous level of total Thymidylate Synthase.

背景

Thymidylate synthase (TS) is a key enzyme in the synthesis of 2'-deoxythymidine-5'-monophosphate, an essential precursor for DNA biosynthesis. In addition to its role in catalysis and cellular metabolism, it also functions as an RNA binding protein. Thymidylate synthase binds with high affinity to its own mRNA resulting in translational repression. TS is involved in a complex pathway that is related to many other folate dependent enzymes; at least three pathways have been identified and completely or partially described. The first essential pathway is the TS cycle, which is devoted to the regeneration of the reduced and active folate form (methylentetrahydrofolate, mTHF), which is necessary for the reaction that leads to dTMP from dUMP. The second TS pathway is related to the feedback regulation of TS expression. The third and most recently identified pathway involving the TS enzyme is that of regulatory TS proteins or rTS.

使用信息

抗体应用

WB,ELISA,ELISA

稀释比例

WB

1:1000

反应性

Human Mouse Rat

MW (kDa)

36kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

Application Data

WB

Validated by Selleck