TOP1 Rabbit Recombinant mAb

生物描述

特异性

TOP1 Rabbit Recombinant mAb detects endogenous level of total TOP1.

背景

DNA topoisomerases regulate the number of topological links between two DNA strands (i.e. change the number of superhelical turns) by catalysing transient single- or double-strand breaks, crossing the strands through one another, then resealing the breaks. They are divided into two classes: type I enzymes (EC; topoisomerases I, III and V) break single-strand DNA, and type II enzymes (EC; topoisomerases II, IV and VI) break double-strand DNA. Top1 (topoisomerase I) regulates DNA topology during replication, transcription, and chromatin remodeling. It removes DNA torsional stress (supercoiling) by forming cleavage complexes (Top1-DNA cleavage complexes [Top1cc's]) in which one DNA strand is cleaved by covalent binding of Top1 to a 3' DNA phosphate. After DNA relaxation, Top1cc's reverse rapidly, and Top1 is released as the DNA religates.

使用信息

抗体应用	WB, IHC, IF,ELISA,ELISA
稀释比例	WB IHC IF 1:1000 1:50 1:50
反应性	Human Mouse
MW (kDa)	91kDa
抗体类型	Rabbit
储存液配方	10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.
储存条件 (自收到货起)	Store at –20°C.

文献参考

[1] Regairaz M, et al. J Cell Biol. 2011, 195(5):739-49. [2] Gadelle D, et al. Bioessays. 2003, 25(3):232-42.

Application Data

WB

IHC

IF