生物描述

特异性

Transferrin Receptor 2 Rabbit Recombinant mAb detects endogenous levels of total Transferrin Receptor 2

背景

The effectiveness of hematopoiesis depends on hematopoietic cell activity as well as on the presence of iron. Iron uptake is regulated by some of the members of the transferrin family of iron-containing proteins -- transferrin and its receptors which assigns them a key regulatory role in hematopoiesis. Transferrins are expressed mainly in the liver, but small amounts arise also in the bone marrow, pancreas, testes, brain, spleen and kidneys. There are three types of transferrin receptors -- transferrin receptor 1 (TfR1), transferrin receptor 2 (TfR2) and soluble transferrin receptor (sTfR). Cell surface TfR expression and concentration reflect iron requirements of the cells and they may be a useful marker for quantitative evaluation of the erythroid lineage, erythropoiesis and iron deficiency. Erythropoietin (Epo) is required for erythroid progenitor differentiation. Type 2 transferrin receptor (TfR2) is a component of the EpoR complex corresponding to proteins. It is involved in iron metabolism by regulating hepcidin production in liver cells. TfR2 and EpoR are synchronously coexpressed during the differentiation of erythroid progenitors. TfR2 associates with EpoR in the endoplasmic reticulum and is required for the efficient transport of this receptor to the cell surface. TfR2 expression is tissue-restricted with high expression in the liver where it plays a key role in iron metabolism regulation. TfR2 contributes to the adaptation of hepcidin production to the needs of the body by sensing the circulating iron bound to transferrin. TfR2 and TfR1 differ in their expression during erythroid differentiation. TfR2 was reduced while TfR1 levels increased with differentiation in MEL cells and in human erythroid cells having the highest TfR2 expression in erythroblasts. TfR2 mRNA expression is cell cycle dependent. Inactivation of TfR2 leads to the development of hemochromatosis due to inappropriate hepcidin levels relative to body iron. TfR2 is suggested to be required forterminal erythroblast differentiation and play a key regulatory role of TfR2 in erythropoiesis.

使用信息

抗体应用	WB, IHC,ELISA,ELISA
稀释比例	WB IHC 1:1000 1:50
反应性	Human
MW (kDa)	100kDa
抗体类型
储存液配方	10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.
储存条件 (自收到货起)	Store at –20°C.

文献参考

[1] Forejtnikovà H, et al. Blood. 2010, 116(24):5357-67.

Application Data

WB

IHC