生物描述

特异性

TRX1 p53 Rabbit Recombinant mAb detects endogenous levels of total TRX1.

背景

Thioredoxin 1 (Trx1) is a conserved antioxidant protein that is well known for its disulfide reductase activity. It is found primarily in the cytosol and occasionally in the nucleus. TRX1 plays critical roles in regulating protein thiol homeostasis and redox signaling both inside and outside the cellular environment. Trx1 regulates a wide range of cellular functions, including cell growth, proliferation, and apoptosis. Its dysfunction is associated with a variety of diseases in which redox imbalance has been implicated, including cancer, human immunodeficiency virus infection, neurodegenerative diseases, and cardiovascular diseases. Under physiological conditions, Trx1 maintains the redox status of proteins via its reductase or denitrosylating activities, sustained with the reducing equivalent from TrxR/NADPH. Within highly oxidative environments, Trx1 becomes S-nitrosylated and offers an alternative modality of protein regulation via transnitrosylation. Transnitrosylation may serve two purposes: to temporarily prevent proteins from acquiring irreversible oxidative modifications when cellular antioxidant systems are overwhelmed, and to serve as a signaling mechanism for altering protein function, contributing to the cellular stress response.

使用信息

抗体应用	WB, IHC,ELISA,ELISA
稀释比例	WB IHC 1:1000 1:50
反应性	Human
MW (kDa)	12kDa
抗体类型
储存液配方	10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.
储存条件 (自收到货起)	Store at –20°C.

文献参考

[1] Changgong Wu, et al. Antioxid Redox Signal. 2011, 15(9): 2565–2604.

Application Data

WB

IHC