Datasheet

生物描述

特异性	USP13 Rabbit Recombinant mAb detects endogenous levels of total USP13.
背景	USP13 (ubiquitin-specific protease 13) is a deubiquitinase belonging to the cysteine-dependent protease superfamily. It functions as a ubiquitin-specific enzyme by cleaving the K48-linked polyubiquitin chain off protein substrate to reverse ubiquitin-mediated protein degradation. USP13 plays important roles in the regulation of specific protein substrates. It has been reported that USP13 can deubiquitinate and regulate protein levels of Beclin-1, microphathalmia-associated transcription factor, Siah2, phosphatase and tensin homolog, and STAT-1, and the deubiquitination process of USP13 could also be orchestrated by Beclin-1. USP13 also interacts with USP10, and the interaction increases USP13 deubiquitinating activity. In addition, USP13 interacts with the P97/valosin-containing protein complex, a key chaperone in ER-associated degradation, which increases the protein level of CD3δ, an ER-associated degradation substrate. USP13 can antagonize gp78 (a ubiquitin E3 ligase) to deubiquitinate Ubl4A, a key component of the Bag6 chaperone complex in ER-associated degradation. USP13 plays an important role in DNA repair and RAP80 is a target of USP13. In response to DNA damage, USP13 is phosphorylated by ATM, which in turn facilitates USP13 recruitment to DSBs, RAP80 deubiquitination, and triggers DDR signalling. Aberrant expression of USP13 has been associated with human cancers.

特异性

USP13 Rabbit Recombinant mAb detects endogenous levels of total USP13.

背景

USP13 (ubiquitin-specific protease 13) is a deubiquitinase belonging to the cysteine-dependent protease superfamily. It functions as a ubiquitin-specific enzyme by cleaving the K48-linked polyubiquitin chain off protein substrate to reverse ubiquitin-mediated protein degradation. USP13 plays important roles in the regulation of specific protein substrates. It has been reported that USP13 can deubiquitinate and regulate protein levels of Beclin-1, microphathalmia-associated transcription factor, Siah2, phosphatase and tensin homolog, and STAT-1, and the deubiquitination process of USP13 could also be orchestrated by Beclin-1. USP13 also interacts with USP10, and the interaction increases USP13 deubiquitinating activity. In addition, USP13 interacts with the P97/valosin-containing protein complex, a key chaperone in ER-associated degradation, which increases the protein level of CD3δ, an ER-associated degradation substrate. USP13 can antagonize gp78 (a ubiquitin E3 ligase) to deubiquitinate Ubl4A, a key component of the Bag6 chaperone complex in ER-associated degradation. USP13 plays an important role in DNA repair and RAP80 is a target of USP13. In response to DNA damage, USP13 is phosphorylated by ATM, which in turn facilitates USP13 recruitment to DSBs, RAP80 deubiquitination, and triggers DDR signalling. Aberrant expression of USP13 has been associated with human cancers.

使用信息

抗体应用

wb,ELISA,ELISA

稀释比例

WB

1:1000

反应性

Human Mouse

MW (kDa)

97kDa

抗体类型

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

Application Data

WB

Validated by Selleck