Datasheet

生物描述

特异性	VCAM1 Rabbit Recombinant mAb detects endogenous levels of total VCAM1.
背景	Vascular cell adhesion molecule-1 (VCAM-1, also known as CD106) is expressed on the luminal and lateral side of endothelial cells under inflammatory conditions, mediating rolling and adhesion of various subsets of leukocytes as prerequisite for recruitment from blood into tissue. Thus it is a keyplayer in the immune surveillance to numerous diseases. VCAM-1 is also expressed on bone marrow stromal cells and therefore involved in the homing of human CD34 early hematological progenitor cells to the bone marrow stroma. VCAM-1 belongs to the immunglobulin (Ig) superfamily of proteins. In activated endothelial cells, VCAM-1 is localized in lipid-raft-like platforms with the tetraspanins CD9, CD81, and CD151, referred as tetraspanin-enriched microdomains, suggested to support VCAM-1 surface expression and function. Beside endothelial cells, it is also expressed on follicular dendritic cells, macrophages in the spleen and thymus, and Kupffer cells in the liver under noninflamed conditions. Because of its wide distribution in human tissues and organs, VCAM-1 participates in a plenty of pathophysiological situations like autoimmune diseases, cardiovascular disease, or infections. VCAM-1 expression could be induced by cytokines produced in the tissue, high levels of ROS, oxidized low density lipoprotein (oxLDL), 25-hydroxycholesterol, turbulent shear stress, high glucose, and microbial stimulation of endothelial cell TLRs. This activation of VCAM-1 gene expression is regulated by the transcription factors nuclear factor kappa B (NFκB), SP-1, Ap-1, and interferon regulatory factor-1. Once VCAM-1 is expressed, it is a scaffold on which leukocytes migrate and also activates signals in endothelial cells that are required for VCAM-1-dependent leukocyte migration.

特异性

VCAM1 Rabbit Recombinant mAb detects endogenous levels of total VCAM1.

背景

Vascular cell adhesion molecule-1 (VCAM-1, also known as CD106) is expressed on the luminal and lateral side of endothelial cells under inflammatory conditions, mediating rolling and adhesion of various subsets of leukocytes as prerequisite for recruitment from blood into tissue. Thus it is a keyplayer in the immune surveillance to numerous diseases. VCAM-1 is also expressed on bone marrow stromal cells and therefore involved in the homing of human CD34 early hematological progenitor cells to the bone marrow stroma. VCAM-1 belongs to the immunglobulin (Ig) superfamily of proteins. In activated endothelial cells, VCAM-1 is localized in lipid-raft-like platforms with the tetraspanins CD9, CD81, and CD151, referred as tetraspanin-enriched microdomains, suggested to support VCAM-1 surface expression and function. Beside endothelial cells, it is also expressed on follicular dendritic cells, macrophages in the spleen and thymus, and Kupffer cells in the liver under noninflamed conditions. Because of its wide distribution in human tissues and organs, VCAM-1 participates in a plenty of pathophysiological situations like autoimmune diseases, cardiovascular disease, or infections. VCAM-1 expression could be induced by cytokines produced in the tissue, high levels of ROS, oxidized low density lipoprotein (oxLDL), 25-hydroxycholesterol, turbulent shear stress, high glucose, and microbial stimulation of endothelial cell TLRs. This activation of VCAM-1 gene expression is regulated by the transcription factors nuclear factor kappa B (NFκB), SP-1, Ap-1, and interferon regulatory factor-1. Once VCAM-1 is expressed, it is a scaffold on which leukocytes migrate and also activates signals in endothelial cells that are required for VCAM-1-dependent leukocyte migration.

使用信息

抗体应用

wb,ELISA,ELISA

稀释比例

WB

1:1000

反应性

Human Mouse Rat

MW (kDa)

100kDa

抗体类型

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

Application Data

WB

Validated by Selleck