生物描述

特异性

Vimentin Rabbit Recombinant mAb detects endogenous levels of total Vimentin.

背景

Vimentin, a major constituent of the intermediate filament (IF) family of proteins, is ubiquitously expressed in normal mesenchymal cells and is known to maintain cellular integrity and provide resistance against stress. It is a marker for epithelial-mesenchymal transition (EMT), potential biomarker for different cancers with clinical relevance, and serves as an attractive target for cancer therapy. During murine development, vimentin expression commences on embryonic day 8.5 and its expression is predominant in the primitive streak stage, while in adults vimentin expression is limited to connective tissue mesenchymal cells, in CNS and in muscle. Increased vimentin expression has been reported in various tumor cell lines and tissues including prostate cancer, breast cancer, endometrial cancer, CNS tumors, malignant melanoma and gastrointestinal tumors including pancreatic, colorectal and hepatic cancers. Vimentin serves as an excellent substrate for a number of kinases in vitro and multiple phosphorylation sites on vimentin have been identified. Phosphorylation of vimentin is associated with functional consequences including the regulation of IF structure and several signaling pathways. Vimentin has also shown to be a target for other types of post-translational modifications including citrullination, sumoylation and O-GlcNAc modification.

使用信息

抗体应用	WB, IF,ELISA,ELISA
稀释比例	WB IF 1:1000 1:50
反应性	Human Mouse Rat
MW (kDa)	54kDa
抗体类型
储存液配方	10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.
储存条件 (自收到货起)	Store at –20°C.

文献参考

[1] Satelli A, et al. Cell Mol Life Sci. 2011, 68(18):3033-46.

Application Data

WB

IHC

IF