YAP1 Rabbit Recombinant mAb

生物描述

特异性

YAP1 Rabbit Recombinant mAb detects endogenous level of total YAP1.

背景

YAP1 is a major effector of the Hippo pathway. The YAP1/Hippo signaling pathway is a key regulator of organ size and tissue homeostasis, and its dysregulation is linked to cancer development. YAP1 activity is mediated predominantly via TEAD transcription factors. YAP1 and TEAD1 exert their transcriptional control via binding to enhancers, leading to characteristic chromatin changes and distal activation of genes. Activation of the Hippo pathway leads to phosphorylation and inactivation of the transcriptional co-activator YAP1 by cytoplasmic retention or enhanced degradation. YAP1 is phosphorylated by the Lats1 tumor suppressor, it translocates to the cytoplasm, where it interacts with 14-3-3 proteins and is thought to be inactive. YAP1 has a potent growth promoting activity and the YAP1/Hippo pathway has been tightly linked to cancer. YAP1 lacks an intrinsic DNA-binding domain and is thought to exert its co-activator function through binding to promoter sequences via interaction with transcription factors (TF), such as TEAD1/-2/-3/-4, Smads, Runx1/-2, p73, ErbB4, Pax3, AP-1, or TBX5.

使用信息

抗体应用	WB, IHC, IF,ELISA,ELISA
稀释比例	WB IHC IF 1:1000 1:50 1:50
反应性	Human
MW (kDa)	65kDa
抗体类型	Rabbit
储存液配方	10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.
储存条件 (自收到货起)	Store at –20°C.

文献参考

[1] Stein C, et al. PLoS Genet. 2015, 11(8):e1005465. [2] Fernandez-L A, et al. Genes Dev. 2009, 23(23):2729-41.

Application Data

WB

IHC

IF