ZAP70 Rabbit Recombinant mAb

生物描述

特异性

ZAP70 Rabbit Recombinant mAb detects endogenous level of total ZAP70.

背景

ZAP-70 is a cytoplasmic protein tyrosine kinase that plays a critical role in the events involved in initiating T-cell responses by the antigen receptor. ZAP-70 contains several tyrosine residues that are phosphorylated following TCR stimulation and are known to be important in its regulation, activity, and association with other signaling molecules. Y292, Y315, and Y319 are located within interdomain B and when phosphorylated are thought to serve as docking sites for downstream signaling molecules. In resting T cells, ZAP-70 is distributed throughout the cytoplasm, but is rapidly recruited to the plasma membrane following TCR stimulation and ITAM phosphorylation. ZAP-70 mobility in the cytoplasm is rapid, and decreases as ZAP-70 associates with the plasma membrane.

使用信息

抗体应用	WB, IHC,ELISA,ELISA
稀释比例	WB IHC 1:1000 1:50
反应性	Human
MW (kDa)	70kDa
抗体类型	Rabbit
储存液配方	10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.
储存条件 (自收到货起)	Store at –20°C.

文献参考

[1] Wang H, et al. Cold Spring Harb Perspect Biol. 2010, 2(5): a002279.

Application Data

WB

IHC