ZNRF2 Rabbit Recombinant mAb Datasheet

生物描述

特异性	ZNRF2 Rabbit Recombinant mAb detects endogenous level of total ZNRF2.
背景	ZNRF2 is the membrane-associated E3 ubiquitin ligase that is released into the cytosol upon its phosphorylation by Akt. It is tethered to intracellular membranes, via an N-myristoyl moiety, where it ubiquitylates the Na+/K+ATPase pump. ZNRF2 is robustly phosphorylated on Ser19, Ser82 and Ser145 in response to growth factors, phorbol ester (PMA) and forskolin. The phosphorylations of Ser19 and Ser145 promote the release of ZNRF2 from intracellular membranes into the cytosol in an Akt-dependent manner. ZNRF2 is a positive regulator of nutrient-mediated mTORC1 signalling and also a negative feedback target of mTORC1 signalling.

特异性

ZNRF2 Rabbit Recombinant mAb detects endogenous level of total ZNRF2.

背景

ZNRF2 is the membrane-associated E3 ubiquitin ligase that is released into the cytosol upon its phosphorylation by Akt. It is tethered to intracellular membranes, via an N-myristoyl moiety, where it ubiquitylates the Na+/K+ATPase pump. ZNRF2 is robustly phosphorylated on Ser19, Ser82 and Ser145 in response to growth factors, phorbol ester (PMA) and forskolin. The phosphorylations of Ser19 and Ser145 promote the release of ZNRF2 from intracellular membranes into the cytosol in an Akt-dependent manner. ZNRF2 is a positive regulator of nutrient-mediated mTORC1 signalling and also a negative feedback target of mTORC1 signalling.

使用信息

抗体应用

WB,ELISA,ELISA

稀释比例

WB

1:1000

反应性

Human Mouse Rat

MW (kDa)

24kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

[1] Hoxhaj G, et al. Elife. 2016, 5. pii: e12278.

Application Data

WB

Validated by Selleck