CRISPR-Cas9 SP Rabbit Recombinant mAb

目录号：A5899

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规格	价格	库存
20ul	RMB 447.24	现货
100ul	RMB 1500.57	现货
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使用信息

抗体应用

wb,ELISA

稀释比例

1:1000

反应性

Streptococcus pyogenes

MW (kDa)

158kDa

抗体类型

Rabbit

浓度

1mg/ml

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

Datasheet & SDS

生物描述

特异性	CRISPR-Cas9 SP Rabbit Recombinant mAb detects endogenous levels of total CRISPR-Cas9 SP.
背景	The CRISPR/Cas9 system has proven to be an efficient gene-editing tool for genome modification of cells and organisms, utilizing an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA; Cas9 only stabilizes the pre-crRNA:tracrRNA interaction and has no catalytic function in RNA processing. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. Cas9 is inactive in the absence of the 2 guide RNAs (gRNA). The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed 3'-5' exonucleolytically. Cas9 is responsible for recognizing the protospacer adjacent motif (PAM) in the CRISPR repeat sequences to help distinguish self versus nonself, as targets within the bacterial CRISPR locus do not have PAMs, and then exerts its endonuclease activity to the genome.

特异性

CRISPR-Cas9 SP Rabbit Recombinant mAb detects endogenous levels of total CRISPR-Cas9 SP.

背景

The CRISPR/Cas9 system has proven to be an efficient gene-editing tool for genome modification of cells and organisms, utilizing an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA; Cas9 only stabilizes the pre-crRNA:tracrRNA interaction and has no catalytic function in RNA processing. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. Cas9 is inactive in the absence of the 2 guide RNAs (gRNA). The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed 3'-5' exonucleolytically. Cas9 is responsible for recognizing the protospacer adjacent motif (PAM) in the CRISPR repeat sequences to help distinguish self versus nonself, as targets within the bacterial CRISPR locus do not have PAMs, and then exerts its endonuclease activity to the genome.

实验方法

参考文献	[1] Jinek M, et al. Science. 2012, 337(6096):816-21. [2] Sternberg SH, et al. Nature. 2014, 507(7490):62-7. [3] Fonfara I, et al. Nucleic Acids Res. 2014, 42(4):2577-90. [4] Jinek M, et al. Science. 2014, 343(6176):1247997. [5] Nishimasu H, et al. Cell. 2014, 156(5):935-49. + Expand

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